中国预防兽医学报
中國預防獸醫學報
중국예방수의학보
CHINESE JOURNAL OF PREVENTIVE VETERINARY MEDICINE
2009年
11期
860-863,909
,共5页
迟佳琦%李闰婷%朱战波%曹宏伟%崔玉东
遲佳琦%李閏婷%硃戰波%曹宏偉%崔玉東
지가기%리윤정%주전파%조굉위%최옥동
奶牛乳房炎%金黄色葡萄球菌%毒力因子%荚膜血清型%PCR检测
奶牛乳房炎%金黃色葡萄毬菌%毒力因子%莢膜血清型%PCR檢測
내우유방염%금황색포도구균%독력인자%협막혈청형%PCR검측
S. aureus%virulence determinants%capsular serotype%PCR-based detection
为研究金黄色葡萄球菌(S.aureus)的因型特征,本实验利用聚合酶链式反(PCR)方法,对S.aureus的3个标准株和30个临床分离株的凝集因子A、凝固酶、溶血素等33种致病因子进行了检测.结果表明各分离株在毒力因子方面存在一定差异.这些差异的检测将为进一步研究S.aureus的致病性和有效防治提供参考.
為研究金黃色葡萄毬菌(S.aureus)的因型特徵,本實驗利用聚閤酶鏈式反(PCR)方法,對S.aureus的3箇標準株和30箇臨床分離株的凝集因子A、凝固酶、溶血素等33種緻病因子進行瞭檢測.結果錶明各分離株在毒力因子方麵存在一定差異.這些差異的檢測將為進一步研究S.aureus的緻病性和有效防治提供參攷.
위연구금황색포도구균(S.aureus)적인형특정,본실험이용취합매련식반(PCR)방법,대S.aureus적3개표준주화30개림상분리주적응집인자A、응고매、용혈소등33충치병인자진행료검측.결과표명각분리주재독력인자방면존재일정차이.저사차이적검측장위진일보연구S.aureus적치병성화유효방치제공삼고.
The present study was carried out to genotypically characterize Staphylococcus aureus strains isolated from bovine mastitis cases. A total of 33 S. Aureus strains were characterized by polymerase chain reaction (PCR) using primers that amplify 31 genes encoding coagulase (coa), clumping factor (clfA), hemolysin, capsular polysaccharides (CP), and other virulence factors. The findings of the present studies would provide better understanding of the distribution of the prevalent S. Aureus clones among bovine mastiffs isolates, therefore aid in the development control measures against S. Aureus infections in dairy herds.
