CAJ | 학술논문

在细胞周期中, 与染色质凝集偶联的一类组蛋白修饰是组蛋白H3的磷酸化.运用H3-Ser 10磷酸化的特异性抗体,通过间接免疫荧光标记检测了磷酸化组蛋白H3在小麦(Triticum aestivum L.)有丝分裂与减数分裂细胞中的分布.有丝分裂时,H3磷酸化起始于早前期,消失于末期,在中期与后期,H3磷酸化主要分布在着丝粒两侧的异染色质区.减数分裂时,H3磷酸化起始于细线期向偶线期转换时,并且从前期Ⅰ到后期Ⅰ保持均一分布于整个染色体上,直到末期Ⅰ消失,而中期Ⅱ与后期Ⅱ在着丝粒两侧的异染色质区的信号略强于染色体臂,直至消失于末期Ⅱ.磷酸化组蛋白H3在两类细胞分裂中的不同分布暗示这种保守的翻译后修饰可能发挥着除参与染色体凝集外的更复杂的作用.
재세포주기중, 여염색질응집우련적일류조단백수식시조단백H3적린산화.운용H3-Ser 10린산화적특이성항체,통과간접면역형광표기검측료린산화조단백H3재소맥(Triticum aestivum L.)유사분렬여감수분렬세포중적분포.유사분렬시,H3린산화기시우조전기,소실우말기,재중기여후기,H3린산화주요분포재착사립량측적이염색질구.감수분렬시,H3린산화기시우세선기향우선기전환시,병차종전기Ⅰ도후기Ⅰ보지균일분포우정개염색체상,직도말기Ⅰ소실,이중기Ⅱ여후기Ⅱ재착사립량측적이염색질구적신호략강우염색체비,직지소실우말기Ⅱ.린산화조단백H3재량류세포분렬중적불동분포암시저충보수적번역후수식가능발휘착제삼여염색체응집외적경복잡적작용.
One of the prominent cell cycle related modifications of histone proteins, whose function is correlated with chromosome condensation, is the phosphorylation of histone H3. Wheat (Triticum aestivum L.) mitotic and meiotic cells were analyzed with indirect immunoflurorescence labeling with an antibody recognizing histone H3 phosphorylated at Serine 10 to study the localization of phosphorylated histone H3 at mitosis and meiosis. Our results showed that, during mitotic division, the phosphorylation of H3 started from early prophase and vanished at telophase, remaining mainly in the pericentromeric regions at metaphase and anaphase. During meiotic division, phosphorylation of H3 initiated at the transition from leptotene to zygotene and remained uniform, along the chromosomes from prophase Ⅰ until telophase Ⅰ, whereas it showed slightly stronger in the pericentromeric regions than along the chromosome arms from metaphase Ⅱ until telophase Ⅱ. The different patterns of H3 phophorylation at mitosis and meiosis in wheat suggested that this evolutionarily conserved post_translational chromatin modification might be involved in more roles besides chromosome condensation.