CAJ | 학술논문

从福建、浙江、湖南、宁夏、安徽、山西、河南、河北、北京等省市采集并收集到16个黄瓜花叶病毒(CMV)样品,用亚组特异性抗血清以ELISA方法对这些样品进行了亚组鉴定.同时,选取亚组I、II病毒部分分离物进行了症状、致病性和体外稳定性比较研究.结果表明,亚组II病毒分离物的症状和致病性受温度、湿度和光照强度等的调节,在不同环境条件下呈现复杂的寄主适应性变化,总的症状比较温和.我国收集的CMV样品以亚组I为主,检出率占样品总数的75%. 但由两亚组病毒生物学特性比较研究结果表明,亚组II病毒在采样和检测中易于被忽略或丢失,据此,探讨了CMV亚组II存在的生态意义及其样品检出率与田间实际发生率之间可能存在的较大差异.
종복건、절강、호남、저하、안휘、산서、하남、하북、북경등성시채집병수집도16개황과화협병독(CMV)양품,용아조특이성항혈청이ELISA방법대저사양품진행료아조감정.동시,선취아조I、II병독부분분리물진행료증상、치병성화체외은정성비교연구.결과표명,아조II병독분리물적증상화치병성수온도、습도화광조강도등적조절,재불동배경조건하정현복잡적기주괄응성변화,총적증상비교온화.아국수집적CMV양품이아조I위주,검출솔점양품총수적75%. 단유량아조병독생물학특성비교연구결과표명,아조II병독재채양화검측중역우피홀략혹주실,거차,탐토료CMV아조II존재적생태의의급기양품검출솔여전간실제발생솔지간가능존재적교대차이.
16 CMV samples from Fujian,Zhejiang, Hunan, Ningxia, Anhui, Shanxi, Henan, Hebei and Binjing were collected and identified by DAS-EIiSA with subgroup specific Antisera. The symptoms, pathygenicities, and in vivo stabil-ities of part of the isolates were compared in Nicotiana tabacum . The results showed that the majority of the CMV samples belong to the subgroup Ⅰ virus, consisting of 75 % of the samples. The symptoms and pathygenicities of subgroup Ⅱ virus were generally mild and flexible, and adjusted by temperature, humidity and light intensity, suggesting its complex flexibil-ity to the host plant. These results suggested that the subgroup Ⅱ virus was prone to be neglected or discarded in sample collecting or experimenting. The differences between detection frequency and actual existence, also the ecological signifi-cance of subgroup Ⅱ virus were discussed.