CAJ | 학술논문

目的筛选分离葛根总黄酮的最佳树脂,并对影响分离的各种因素进行系统的研究,使纯化工艺达到最优.方法采用静态与动态的吸附-解吸两种方法,利用紫外可见分光光度计测量葛根总黄酮的含量,研究不同大孔吸附树脂及其不同的工艺条件对总黄酮分离纯化的影响.结果SP70分离效果最好,其最佳工艺为药液浓度0.5 g·mL-1(相当于原生药)、pH为5-6、以2 BV·h-1速率进行上样,上样量为60BV,以5 BV的70%乙醇、2 BV·h-1的流速进行洗脱,效果最佳.经SP70处理后的葛根总黄酮的含量可达80%以上.结论大孔吸附树脂SP70分离纯化总黄酮效果较好,适合工业生产.
목적사선분리갈근총황동적최가수지,병대영향분리적각충인소진행계통적연구,사순화공예체도최우.방법채용정태여동태적흡부-해흡량충방법,이용자외가견분광광도계측량갈근총황동적함량,연구불동대공흡부수지급기불동적공예조건대총황동분리순화적영향.결과SP70분리효과최호,기최가공예위약액농도0.5 g·mL-1(상당우원생약)、pH위5-6、이2 BV·h-1속솔진행상양,상양량위60BV,이5 BV적70%을순、2 BV·h-1적류속진행세탈,효과최가.경SP70처리후적갈근총황동적함량가체80%이상.결론대공흡부수지SP70분리순화총황동효과교호,괄합공업생산.
Aim To screen the optimum macroporous resin and conditions for the isolation and purification of flavonoids from Radix Puerariae. Methods The static and dynamic adsorption/desorption methods were used, and the separation and purification process was evaluated by measuring the concentration of total flavonoid in the fractions with UV spectrophotometer. Results The SP70 macroporous resin was the most effective compared with other macroporous resins. The optimum conditions were screened, which were 0. 5 g· mL- 1 corresponding to crude drug for concentration of extract, pH 5 - 6, and appended 60 times the volume of the resin bed (BV) with the adsorption speed 2 BV· h - 1,and the volume of aq. 70％ (V/V) ethanol as eluant was 5 BV with desorption speed 2 BV·h-1. By this method, the final contents of total flavonoids exceeded 80%. Conclusion The SP70 macroporousresin is the most effective one for large-scale isolation and purification of flavonoids from Radix Pueraria, which meets industrial needs.