CAJ | 학술논문

在对杂交品种741杨(Populus alba(P.davidiana×P.simonii)×P.tomentosa)进行农杆菌介导法转基因的试验中发现了一种快速有效的叶片再生芽的方法.首先叶片外植体在培养基Ⅰ(MS培养基添加0.5 mg/L BA和1.0 mg/L 2,4-D)上培养2～3 d,再转移到培养基SH(MSmedium containing 2.0 mg/L of BA and 0.1 mg/L of NAA)上培养10 d,然后再转移到培养基Ⅱ(MSmedium with 0.5 mg/L of BA)上,培养大约5 d之后86.7%的叶片外植体产生的芽,每片叶片外植体(1 cm×1 cm)可产生40～50个芽.但是,如果叶片外植体在培养基Ⅰ上培养的时间长于5 d,再依次转移到培养基SH和Ⅱ上,则叶片会产生大量根.
재대잡교품충741양(Populus alba(P.davidiana×P.simonii)×P.tomentosa)진행농간균개도법전기인적시험중발현료일충쾌속유효적협편재생아적방법.수선협편외식체재배양기Ⅰ(MS배양기첨가0.5 mg/L BA화1.0 mg/L 2,4-D)상배양2～3 d,재전이도배양기SH(MSmedium containing 2.0 mg/L of BA and 0.1 mg/L of NAA)상배양10 d,연후재전이도배양기Ⅱ(MSmedium with 0.5 mg/L of BA)상,배양대약5 d지후86.7%적협편외식체산생적아,매편협편외식체(1 cm×1 cm)가산생40～50개아.단시,여과협편외식체재배양기Ⅰ상배양적시간장우5 d,재의차전이도배양기SH화Ⅱ상,칙협편회산생대량근.
In the experiments of Agrobacterium tumefaciens-mediated transformation of the hybrid poplar 741, Populus alba ( P. davidiana × P. simonii) × P. tomentosa, we developed a rapid and efficient method of shoot regeneration from the leaf explants in-vitro on Murashige and Skoog (MS) medium. The leaf explant segments was first cultured on medium Ⅰ ( MS medium supplemented with 0.5 mg/L of BA and 1.0 mg/L of 2,4-D ) for 2 ～ 3 days and then transferred to medium SH ( MS medium containing 2.0mg/L of BA and 0. 1 mg/L of NAA) to culture for 10 days. 86.7% of leaf segments produced shoots after the leaf segments were transferred from medium SH to medium Ⅱ (MS medium with 0.5 mg/L of BA) for 1 week. The average shooting number was 40 ～ 50 per 1 cm × 1 cm of leaf segment. However, if the leaf explants incubated on medium Ⅰ longer than 5 days, they produced more roots instead of shoots.