CAJ | 학술논문

提出了一种改进的SDS-PAGE方法. 采用0.46mol/L Tris-0.047mol/L glycine (pH?9.57)+1.0g/L SDS连续缓冲体系，分离胶T=15%(C=3%)和浓缩胶T=4%(C=3%)时在3.5～68.0?kD分子量范围内具有良好的线性关系，相关系数r=0.94. 该法操作简便分离效果好，使用常用的电泳试剂，适合于蛋白质酶解产物和一些疾病等的小分子多肽分析.
제출료일충개진적SDS-PAGE방법. 채용0.46mol/L Tris-0.047mol/L glycine (pH?9.57)+1.0g/L SDS련속완충체계，분리효T=15%(C=3%)화농축효T=4%(C=3%)시재3.5～68.0?kD분자량범위내구유량호적선성관계，상관계수r=0.94. 해법조작간편분리효과호，사용상용적전영시제，괄합우단백질매해산물화일사질병등적소분자다태분석.
An improved SDS-PAGE method for the separation of low molecular weight polypeptides is studied in this paper. A continuous buffer of 0.46?mol/L Tris-0.047?mol/L glycine (pH?9.57)+1.0?g SDS，is used as buffer for both separation gel and stacking gel as well as electrode buffer in our method. Polypeptides with molecular weights from 3.5 to 68.0?kD can be satisfactorily separated with good linearity (correlation coefficient r=0.94) between the relative mobility and the logarithm of molecular weight. It is a SDS-PAGE method with the advantage of being the most widely used one with greater ease and lower cost， and it is very useful for the study of enzymatic cleavage products and diseases.