高等学校化学学报
高等學校化學學報
고등학교화학학보
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES
2001年
4期
566-568
,共3页
龙远德%王群标%HUANG Tian-Bao
龍遠德%王群標%HUANG Tian-Bao
룡원덕%왕군표%HUANG Tian-Bao
手性配体交换色谱%手性固定相%异喹啉衍生物%DL-氨基酸
手性配體交換色譜%手性固定相%異喹啉衍生物%DL-氨基痠
수성배체교환색보%수성고정상%이규람연생물%DL-안기산
A novel chiral bonded stationary phase(CBSP) for ligand exchange chromatography was prepared by bonding (S)-1,2,3,4-tetrahydro-3-isoquinoline carboxylic acid prepared from L-Phe to YWG-80 silica gel via 3-glycidyloxypropyltrimethoxysilane as a coupling agent. Chromatographic resolutions of some DL-amino acids were achieved on the CBSP by using an aqueous solution of 2 mmol/L N(C2H5)3, 2 mmol/L HAc and 0.2 mmol/L Cu(Ac)2 as the mobile phase with a flow rate 1.0 mL/min, column temperature 50 ℃ and detection at 254 nm. The enantioselectivity α of the DL-amino acids on the CBSP was found to be between 1.11 and 1.51. The elution order of D-isomer before L-isomer on the CBSP was observed for all the DL-amino acids resolved except DL-Val. For DL-Pro, DL-Val and DL-Leu the elution order through the CBSP was different from that through the chiral ligand exchange phases prepared from L-Pro or L-hydroxyl proline with a five-membered ring structure.
