CAJ | 학술논문

探讨自制的改良布氏活性炭巧克力培养基,置厌氧罐蜡烛培养环境分离临床粘膜标本幽门螺杆菌(Hp)的检出率.结果表明:66例粘膜标本中分离出Hp54株(81.8%),44株Hp滤液有24株(54.5%)具有细胞毒活性,其中26例消化性溃疡患者有18例(69.2%),18例慢性胃炎患者有6例(33.3%)为Hp(Toxin+)感染. 结果提示:通过对Hp生物学性状和毒素的检测结果证实,该法可用于临床粘膜标本Hp的常规分离培养.
탐토자제적개량포씨활성탄교극력배양기,치염양관사충배양배경분리림상점막표본유문라간균(Hp)적검출솔.결과표명:66례점막표본중분리출Hp54주(81.8%),44주Hp려액유24주(54.5%)구유세포독활성,기중26례소화성궤양환자유18례(69.2%),18례만성위염환자유6례(33.3%)위Hp(Toxin+)감염. 결과제시:통과대Hp생물학성상화독소적검측결과증실,해법가용우림상점막표본Hp적상규분리배양.
The home-made modified Brucella activated charcoal chocolate agar was put into an anaerobic can candle culture condition to detect the rate of Helicobacter pylori(Hp) of isolating culture from biopsied mucosa samples. The results showed that 54 strains Hp were isolated from 66 mucasa samples (81.8 %). A total of 24 (54.5 %) out of 44 Hp filtrates produced a cytotoxic activity against human endothelium cell in vitro. Hp (Toxin+) infection existed in 18 cases (69.2 %) out of 26 patients with digestive ulcer and 6 (33.3 %) out of 18 patients with chronic gastritis, respectively. The study showed that it is confirmed through the measurement of biologic characters and cytotoxic activity of the isolated Hp that improvement of isolation and culture technique was an effective method and could be used as routinely isolating culture of Hp for biopsy mucosa samples.