生命科学研究
生命科學研究
생명과학연구
LIFE SCIENCE RESEARCH
2001年
2期
146-148
,共3页
紫杉醇%前体%果糖%细胞培养
紫杉醇%前體%果糖%細胞培養
자삼순%전체%과당%세포배양
研究了果糖和几种前体物质对东北红豆杉生产紫杉醇的影响.结果表明, 在第12 d加入6 g/L果糖可以使紫杉醇产量增加63.89%.在有果糖协同的作用下,加入前体(0.05 mmol/L乙酸钠,0.05 mmol/L苯丙氨酸 ,0.1 mmol/L苯甲酸钠)可显著提高紫杉醇的合成,同对照相比,含量分别增加49.36%、13. 18%和64.26%.在第15 d向培养基中加入0.05 mmol/L乙酸钠、0.1 mmol/L苯甲酸钠、1 mmol /L苯丙氨酸和6 g/L果糖则使紫杉醇含量提高181.89%.
研究瞭果糖和幾種前體物質對東北紅豆杉生產紫杉醇的影響.結果錶明, 在第12 d加入6 g/L果糖可以使紫杉醇產量增加63.89%.在有果糖協同的作用下,加入前體(0.05 mmol/L乙痠鈉,0.05 mmol/L苯丙氨痠 ,0.1 mmol/L苯甲痠鈉)可顯著提高紫杉醇的閤成,同對照相比,含量分彆增加49.36%、13. 18%和64.26%.在第15 d嚮培養基中加入0.05 mmol/L乙痠鈉、0.1 mmol/L苯甲痠鈉、1 mmol /L苯丙氨痠和6 g/L果糖則使紫杉醇含量提高181.89%.
연구료과당화궤충전체물질대동북홍두삼생산자삼순적영향.결과표명, 재제12 d가입6 g/L과당가이사자삼순산량증가63.89%.재유과당협동적작용하,가입전체(0.05 mmol/L을산납,0.05 mmol/L분병안산 ,0.1 mmol/L분갑산납)가현저제고자삼순적합성,동대조상비,함량분별증가49.36%、13. 18%화64.26%.재제15 d향배양기중가입0.05 mmol/L을산납、0.1 mmol/L분갑산납、1 mmol /L분병안산화6 g/L과당칙사자삼순함량제고181.89%.
The effects o f some precursors on production of taxol in cell suspensions of T. cuspidata wer e studied. The results showed that the addition of 6 g/L fructose into the cultu res on the 12th day of culture increased taxol yield by 63.89%; the addition of 0.05 mmol/L acetate, 0.05 mmol/L phenylalanine,0.1 mmol/L benzoate with fruct ose ,increased taxol yields by 49.36%,13.18% and 64.26 %,respectively. However, the addition of 0.05 mmol/L acetate , 1 mmol/L phenylalanine,0.1 mmol/L benzoate and 6 g/L fructose into the cultu res on the 15th day of culture dramatically increased taxol yield by 181.89%.
