山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2001年
3期
6-7
,共2页
温明春%杜继斌%魏春华%胡兆秋%曲春枫
溫明春%杜繼斌%魏春華%鬍兆鞦%麯春楓
온명춘%두계빈%위춘화%호조추%곡춘풍
白细胞介素-12%免疫球蛋白E%哮喘%Th1/Th2%应答
白細胞介素-12%免疫毬蛋白E%哮喘%Th1/Th2%應答
백세포개소-12%면역구단백E%효천%Th1/Th2%응답
IL-12 IgE Asthma Th1/Th2 Responsion
为探讨哮喘发作期患者血清白细胞介素-12(IL-12)与免疫球蛋白E(IgE)的关系及其在哮喘发作中的作用,采用双抗体夹心ELISA方法检测了27例哮喘发作期患者血清中的IL-12和IgE水平,并采用SPSS统计分析软件分析了两者之间的关系。结果显示,27例患者血清中IL-12的平均含量(71.70±81.43pg/ml)显著低于正常对照组(196.32±94.33pg/ml),P<0.01;IgE含量(2078.63±611.79ng/ml)显著高于对照组(327.16±125.28ng/ml),P<0.001。统计分析表明,IL-12降低与IgE升高有显著相关性。提示患者体内IL-12产生量不足,可能阻碍了Th1细胞的分化发育,引起了偏离的Th2型的免疫应答,导致超敏反应发生。
為探討哮喘髮作期患者血清白細胞介素-12(IL-12)與免疫毬蛋白E(IgE)的關繫及其在哮喘髮作中的作用,採用雙抗體夾心ELISA方法檢測瞭27例哮喘髮作期患者血清中的IL-12和IgE水平,併採用SPSS統計分析軟件分析瞭兩者之間的關繫。結果顯示,27例患者血清中IL-12的平均含量(71.70±81.43pg/ml)顯著低于正常對照組(196.32±94.33pg/ml),P<0.01;IgE含量(2078.63±611.79ng/ml)顯著高于對照組(327.16±125.28ng/ml),P<0.001。統計分析錶明,IL-12降低與IgE升高有顯著相關性。提示患者體內IL-12產生量不足,可能阻礙瞭Th1細胞的分化髮育,引起瞭偏離的Th2型的免疫應答,導緻超敏反應髮生。
위탐토효천발작기환자혈청백세포개소-12(IL-12)여면역구단백E(IgE)적관계급기재효천발작중적작용,채용쌍항체협심ELISA방법검측료27례효천발작기환자혈청중적IL-12화IgE수평,병채용SPSS통계분석연건분석료량자지간적관계。결과현시,27례환자혈청중IL-12적평균함량(71.70±81.43pg/ml)현저저우정상대조조(196.32±94.33pg/ml),P<0.01;IgE함량(2078.63±611.79ng/ml)현저고우대조조(327.16±125.28ng/ml),P<0.001。통계분석표명,IL-12강저여IgE승고유현저상관성。제시환자체내IL-12산생량불족,가능조애료Th1세포적분화발육,인기료편리적Th2형적면역응답,도치초민반응발생。
To evaluate the relationship and the clinical significancebetween the serum IL-12 and IgE in the patients with asthma in attack.Sandwich ELISA was used to detect the IL-12 and IgE level in the sera of 27 cases of patients with athma in attack.SPSS statical software was used to analysis the reletive of IL-12 and IgE.Results showed that the serum IL-12 in 27 cases of patients with asthma in attack was 71.70±81.43pg/ml,it decresed significantly comparing with the health voluteer donor,which is 196.32±94.33pg/ml.To the contrast,serum IgE in patients was 2078.63±611.79pg/ml,which significantly increaed comparing with the normal(327.16±125.28pg/ml).Linear regression showed that the decrease of IL-12 relate to the increase of IgE(R=-0.667).The results suggest that insufficient production of IL-12 imped the differenciation of Th1.Biased Th2 response may be dominat in the immune response of the asthma in attack.