CAJ | 학술논문

目的　确定活体人胰腺组织是否存在乙型肝炎病毒感染，并探讨其与糖尿病可能存在的关系。方法　手术获取12例血清HBV标志物呈阳性病人的胰腺组织，以原位杂交、免疫组织化学和石蜡切片检测观察。结果　胰腺组织HBV DNA阳性率8/12，其中口服葡萄糖耐量试验(OGTT)异常的7例检测结果均为阳性，OGTT正常的5例中仅1例阳性，HBV DNA主要位于胰腺和胰岛细胞核内。免疫组化检测胰腺HBsAg阳性率为10/12，主要分布在细胞浆。结论　HBV可直接侵害胰腺腺泡和胰岛细胞，是造成胰腺功能异常和糖尿病的可能原因。
목적　학정활체인이선조직시부존재을형간염병독감염，병탐토기여당뇨병가능존재적관계。방법　수술획취12례혈청HBV표지물정양성병인적이선조직，이원위잡교、면역조직화학화석사절편검측관찰。결과　이선조직HBV DNA양성솔8/12，기중구복포도당내량시험(OGTT)이상적7례검측결과균위양성，OGTT정상적5례중부1례양성，HBV DNA주요위우이선화이도세포핵내。면역조화검측이선HBsAg양성솔위10/12，주요분포재세포장。결론　HBV가직접침해이선선포화이도세포，시조성이선공능이상화당뇨병적가능원인。
Objective　To determine whether the hapatitis B virus infection occur in living-body pancreas and its relationship with diabetes mellitus. Method　12 specimens of pancreatic tissue were obtained in patients with positive serum HBV markers. HBV DNA in pancreatic tissue were detected in situ hybridization, by immunohistochemistry and electronic microscopy. Result　8 of 12 pancreatic specimens were positve of HBV DNA. All 7 patients with abnormal oral glucose tolerance test (OGTT) were positive, but only one specimen was positive in patients with normal OGTT. HBV DNA was mainly localized in the mucler of pancreatic acinar and islet cells. The distribution of HBsAg which was detected by immunohistochemical staining was positive in the cytoplasm of pancreatic cells of 10 patients. Conclusion　HBV may not only invade the pancreatic acinar and islet cells directly, but also may be a reason of diabetes mellitus complicated by HBV infection.