CAJ | 학술논문

实验用鸟经脑灌流后于HVc处取材,用Epon 812包埋剂浸润包埋,经铀-铅电子染色后,H-600投射电镜下观察,IBAS图像分析仪进行处理.结果发现,鸣禽HVc核团的神经细胞以圆形、卵圆形为主,胞体体积无明显的种间差别,但雄鸟细胞比雌鸟大. HVc的突触连接以轴-树突触和轴-棘突触为常见.经统计学t检验, HVc单位面积突触总数、I型和II型突触所占比例、棘突触所占比例的种间差异不显著(P>0.05),而性别差异显著(P<0.05).结果表明,鸣禽鸣唱控制核团体积的性别差异不仅反映在显微水平,而且在亚显微水平亦表现出明显的性别二态性.
실험용조경뇌관류후우HVc처취재,용Epon 812포매제침윤포매,경유-연전자염색후,H-600투사전경하관찰,IBAS도상분석의진행처리.결과발현,명금HVc핵단적신경세포이원형、란원형위주,포체체적무명현적충간차별,단웅조세포비자조대. HVc적돌촉련접이축-수돌촉화축-극돌촉위상견.경통계학t검험, HVc단위면적돌촉총수、I형화II형돌촉소점비례、극돌촉소점비례적충간차이불현저(P>0.05),이성별차이현저(P<0.05).결과표명,명금명창공제핵단체적적성별차이불부반영재현미수평,이차재아현미수평역표현출명현적성별이태성.
The bird brains were perfuse and HVc (the caudial nucleus of the ventral hyperstriatum) samples were cut and embedded in Epon 812.The ultrathinsections were mounted on 200-mesh grids,double-stained with ethanolic uranyl acetate, followed by concentrated lead citrate, and examined in H-600 electron microscope. The photographs were treated on IBAS system. The results showed that the size of HVc cells is larger in male than that of in female. But there is no significant difference between species. Axo-dendritic and axo-spinal synapses in HVc are dominant. The statistical test showed that there is no significant difference in the number of the total synapses, Gray I synapses and spinal synapse in HVc between two species (P>0.05), but there is significant difference between sexes (P<0.05).Results suggested that there is sexual dimorphism in ultrastructure of the forebrain HVc. The results provide ultrastructural evidence for the study of sex difference of song behavior of birds.