CAJ | 학술논문

目的：应用内毒素(LPS)所致小鼠全身炎症反应综合征(SIRS)、多器官功能失常综合征(MODS)动物模型，研究参麦注射液对SIRS、MODS的保护作用。方法：将雄性、5～7周龄BALB/c小鼠随机分为正常组、LPS模型组和参麦注射液加LPS组。根据SIRS和MODS临床诊断标准，检查小鼠肛温、外周血白细胞计数、血液生化及肺脏和肝脏病理学，并测定血浆内毒素和血清肿瘤坏死因子(TNFα)水平。结果：腹腔注射LPS可致内毒素血症和高TNFα血症；可使小鼠体温明显降低，外周血白细胞数减少；能明显降低血糖水平，增加血清甘油三酯和胆固醇水平。病理结果显示，LPS可致肺和肝脏毛细血管扩张、充血、水肿和炎细胞浸润。参麦注射液可明显升高LPS所致小鼠的低体温和低外周血白细胞数；可改善内毒素所致小鼠低血糖状态和减轻LPS所致的各脏器病理改变；可明显降低血浆内毒素和TNFα水平。结论：参麦注射液对LPS所致小鼠SIRS和MODS具有显著的保护作用。其保护机制可能与降低内毒素水平和减少TNFα分泌有关。
목적：응용내독소(LPS)소치소서전신염증반응종합정(SIRS)、다기관공능실상종합정(MODS)동물모형，연구삼맥주사액대SIRS、MODS적보호작용。방법：장웅성、5～7주령BALB/c소서수궤분위정상조、LPS모형조화삼맥주사액가LPS조。근거SIRS화MODS림상진단표준，검사소서항온、외주혈백세포계수、혈액생화급폐장화간장병이학，병측정혈장내독소화혈청종류배사인자(TNFα)수평。결과：복강주사LPS가치내독소혈증화고TNFα혈증；가사소서체온명현강저，외주혈백세포수감소；능명현강저혈당수평，증가혈청감유삼지화담고순수평。병리결과현시，LPS가치폐화간장모세혈관확장、충혈、수종화염세포침윤。삼맥주사액가명현승고LPS소치소서적저체온화저외주혈백세포수；가개선내독소소치소서저혈당상태화감경LPS소치적각장기병리개변；가명현강저혈장내독소화TNFα수평。결론：삼맥주사액대LPS소치소서SIRS화MODS구유현저적보호작용。기보호궤제가능여강저내독소수평화감소TNFα분비유관。
Objective: To study the protective effect of Shenmai injection(SMI) on systemic inflammatory reaction syndrome (SIRS) and multiple organ dysfunction syndrome (MODS) in animal model of SIRS、MODS induced by lipopolysaccharide (LPS). Methods: Male BALB/c mice, 5～7 weeks old were randomly divided into 3 groups, the normal control group, the LPS model group and the LPS ＋SMI group. The rectal temperature, peripheral white blood cell count (WBC), blood biochemical examination, histopathologic changes of lung and liver as well as the blood levels of endotoxin and tumor necrosis factor α (TNFα) were determined before and after treatment. Results: LPS could induce endotoxemia and high serum level of TNFα, decrease the rectal temperature and WBC, reduce blood glucose and increase serum triglyceride and cholesterol levels. Pathological examination showed that LPS could cause pulmonary alveolar congestion, edema, exudation, capillary dilation and inflammatory cell infiltration in liver and lung tissue. SMI could significantly raise the low body temperature caused by LPS and reduction of WBC, improve the hypoglycemia and high plasma TNFα level, alleviate the pathologic changes in organs and reduce the plasma level of LPS (endotoxin). Conclusion: SMI has marked effect in protecting LPS caused SIRS、MODS, the mechanism might be related with the lowering of LPS (endotoxin) level and reducing of TNFα secretion.