CAJ | 학술논문

目的构建了三种表达形式的弓形虫主要表面抗原(SAG1)基因-表膜型、分泌型和细胞内型,并分别用于免疫BALB/C小鼠.结果SAG1基因免疫小鼠后,表膜型和分泌型较细胞内型体液免疫出现早且反应强;细胞因子水平测试显示免疫后小鼠免疫反应趋向Th1型.攻击感染结果证实:表膜型和分泌型免疫小鼠较细胞内型免疫小鼠所获保护力强.结论三种表达形式的SAG1基因在小鼠模型中免疫效果不同.
목적구건료삼충표체형식적궁형충주요표면항원(SAG1)기인-표막형、분비형화세포내형,병분별용우면역BALB/C소서.결과SAG1기인면역소서후,표막형화분비형교세포내형체액면역출현조차반응강;세포인자수평측시현시면역후소서면역반응추향Th1형.공격감염결과증실:표막형화분비형면역소서교세포내형면역소서소획보호력강.결론삼충표체형식적SAG1기인재소서모형중면역효과불동.
Three forms of the major surface antigen (SAG1)of Toxoplasma gondii, that is the membrane form, the secrete form and the intracellular form, were constructed and used to immunize BALB/c mice. The humoral response in the mice immunized with the membrane form and the secrete form of SAG1 appeared earlier and stronger than those mice immunized with the intracellular form. Result from the challenging infection demonstrated that the protection in the mice immunized with the membrane and the secrete forms was also stronger than in the mice immunized with the intracellular form. We suggest that the immune efficiency of the three forms of SAG1 in the mouse model is different.