CAJ | 학술논문

目的：研究肿瘤坏死因子-α(TNF-α)在中耳胆脂瘤组织中的表达，探讨其在中耳胆脂瘤骨质破坏中的作用。方法：采用免疫组织化学SABC染色法和计算机图像定量分析法，对25例中耳胆脂瘤和10例正常外耳道皮肤中TNF-α的表达情况进行观察。结果：TNF-α不仅在胆脂瘤上皮的各层细胞胞浆中，而且在皮下组织的炎性细胞中都有高度表达。定量分析显示胆脂瘤上皮中TNF-α的含量显著高于外耳道皮肤(P＜0.05)。结论：TNF-α可能是胆脂瘤引起骨质破坏的一个因素。
목적：연구종류배사인자-α(TNF-α)재중이담지류조직중적표체，탐토기재중이담지류골질파배중적작용。방법：채용면역조직화학SABC염색법화계산궤도상정량분석법，대25례중이담지류화10례정상외이도피부중TNF-α적표체정황진행관찰。결과：TNF-α불부재담지류상피적각층세포포장중，이차재피하조직적염성세포중도유고도표체。정량분석현시담지류상피중TNF-α적함량현저고우외이도피부(P＜0.05)。결론：TNF-α가능시담지류인기골질파배적일개인소。
Objective:To study the expression of TNF-α in the cholesteatoma and elucidate the role of TNF-α in the destruction of bone.Method:All samples (5 μm paraffin sections) from 25 cholesteatoma cases and 10 normal cases were examined by immunohistochemical SABC method and analysed by computer image.Result:In 25 cholesteatoma cases,TNF-α expressed in the cytoplasm of the full epithelial tissue cells,subcutaneous inflammatory cells and fibroblast.In contrast,the normal cases,there were 6 cases week positive,4 cases negative.The results of the computer image quantitative analysis system showed that the mean optical density of TNF-α was 0.1326±0.0022 in the cholesteatoma epithelial tissue and 0.0868±0.0014 in normal skin epithelial tissue respectively,both of which had significantly differentiation (P＜0.05).Conclusion:TNF-α may be a factor of the destruction of bone of cholesteatoma.