中国海洋湖沼学报(英文版)
中國海洋湖沼學報(英文版)
중국해양호소학보(영문판)
Chinese Journal of OCEANOLOGY and LIMNOLOGY
2003年
2期
141-148
,共8页
赫英俊%邹俞萍%王晓东%郑治国%张大明%段德麟
赫英俊%鄒俞萍%王曉東%鄭治國%張大明%段德麟
혁영준%추유평%왕효동%정치국%장대명%단덕린
Laminaria%gametophyte%RAPD%germplasm identification
Eighteen gametophytes including L. japonica, L. ochotensis and L. longissima , were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains of Laminaria , but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species of Laminaria.
