现代医院
現代醫院
현대의원
MODERN HOSPITAL
2009年
8期
15-16
,共2页
陈凯佳%肖莹%刘小斌%何婉婉%庞震苗
陳凱佳%肖瑩%劉小斌%何婉婉%龐震苗
진개가%초형%류소빈%하완완%방진묘
强肌健力口服液%骨髓间充质干细胞%肌凝蛋白%结蛋白
彊肌健力口服液%骨髓間充質榦細胞%肌凝蛋白%結蛋白
강기건력구복액%골수간충질간세포%기응단백%결단백
Serum containing Qiangjijianli oral fluid%Mesenchymal stem cells%Myosin%Desmin
目的 观察强肌健力口服液含药血清对大鼠骨髓间充质干细胞(MSCs)成肌分化的影响.方法 用密度梯度离心法分离大鼠MSCs,5-溴脱氧嘧啶(Brdu)和CD44双重免疫组化鉴定,传3代后的MSCs分为强肌健力口服液含药血清组和空白对照组,免疫组化染色鉴定肌凝蛋白(Myosin)、结蛋白(Desmin)的表达.结果 诱导后第3 d,部分细胞出现Desmin和Myosin.结论 强肌健力口服液含药血清诱导MSCs向成肌细胞定向分化.
目的 觀察彊肌健力口服液含藥血清對大鼠骨髓間充質榦細胞(MSCs)成肌分化的影響.方法 用密度梯度離心法分離大鼠MSCs,5-溴脫氧嘧啶(Brdu)和CD44雙重免疫組化鑒定,傳3代後的MSCs分為彊肌健力口服液含藥血清組和空白對照組,免疫組化染色鑒定肌凝蛋白(Myosin)、結蛋白(Desmin)的錶達.結果 誘導後第3 d,部分細胞齣現Desmin和Myosin.結論 彊肌健力口服液含藥血清誘導MSCs嚮成肌細胞定嚮分化.
목적 관찰강기건력구복액함약혈청대대서골수간충질간세포(MSCs)성기분화적영향.방법 용밀도제도리심법분리대서MSCs,5-추탈양밀정(Brdu)화CD44쌍중면역조화감정,전3대후적MSCs분위강기건력구복액함약혈청조화공백대조조,면역조화염색감정기응단백(Myosin)、결단백(Desmin)적표체.결과 유도후제3 d,부분세포출현Desmin화Myosin.결론 강기건력구복액함약혈청유도MSCs향성기세포정향분화.
Objective To investigate the effects of mesenchymal stem cells(MSCs) differentiation into myoblasts induced by serum containing Qiangjijianli oral fluid.Methods Rat mesenchymal stem cells dissociated from the bone marrow by density gradient method were cultured.MSCs were identified by marking of bromodeoxyuridine (Brdu) and staining of CD44.Experimental group was cultivated with Serum Containing Qiangjijianli oral fluid and control group with blank serum.The antigen expressions of desmin and myosin were evaluated with immunohistochemistry method.Results After induced by serum containing Qiangjijianli oral fluid, some of MSCs expressed desmin and myosin at the 3th day.Conclusion Serum containing Qiangjijianli oral fluid can induce orientational differentiation of MSCs into myoblasts.
