CAJ | 학술논문

目的了解性病门诊患者解脲脲原体(Uu)分离株的血清分型与分子分群的分布状况与相互关系.方法采用液体加固体的方法从临床标本中分离获得解脲脲原体临床菌株,纯化后采用PCR扩增Uu多条带基因进行分子分群,采用代谢抑制试验进行血清分型.结果从377例性病门诊患者中分离到Uu 117例,阳性率31%.其中男性54例,阳性率46%;女性63例,阳性率54%.成功进行分子分群114例,其中生物1群47例,占41.2%;生物2群67例,占58.8%;进行血清分型60例,其中单个型别41例,占68.3%;多型别合并19例,占31.7%.出现频率高的型别为7型24例(29.63%),2型19例(23.46%),14型11例(13.58%)和4型7例(8.64%).未见3、5、12、13 4种血清型别.16.7%Uu分离株(10/60)存在分型与分群结果不符情况.结论性病门诊患者Uu分离株中,生物2群所占比例多于生物1群,血清分型以7、2、14、4等型别常见,发现部分菌株分子分群与血清分型结果不符现象.
목적 료해성병문진환자해뇨뇨원체(Uu)분리주적혈청분형여분자분군적분포상황여상호관계.방법 채용액체가고체적방법종림상표본중분리획득해뇨뇨원체림상균주,순화후채용PCR확증Uu다조대기인진행분자분군,채용대사억제시험진행혈청분형.결과 종377례성병문진환자중분리도Uu 117례,양성솔31%.기중남성54례,양성솔46%;녀성63례,양성솔54%.성공진행분자분군114례,기중생물1군47례,점41.2%;생물2군67례,점58.8%;진행혈청분형60례,기중단개형별41례,점68.3%;다형별합병19례,점31.7%.출현빈솔고적형별위7형24례(29.63%),2형19례(23.46%),14형11례(13.58%)화4형7례(8.64%).미견3、5、12、13 4충혈청형별.16.7%Uu분리주(10/60)존재분형여분군결과불부정황.결론 성병문진환자Uu분리주중,생물2군소점비례다우생물1군,혈청분형이7、2、14、4등형별상견,발현부분균주분자분군여혈청분형결과불부현상.
Objective To investigate the correlation and distribution of biovar and serotype of Ureaplasma urealyticum(Uu) isolates in out-patients with sexually transmitted disease(STD). Methods The liquid culture and solid culture were applied to isolating Uu strains from the clinical specimens. Af-ter purification of Uu strains, the multiple banded antigen gene of Uu was amplified by using polymer-ase chain reaction(PCR),and then the biovar of Uu isolates was performed. The serotype was deter-mined with metabolic inhibition test. Results Totally 117 cases(31%) were Uu positive from 377 clin-ical specimens of STD out-patients,Among them,54 cases(46%) were isolated from males,and 63 ca-ses(54%) were from females. A total of 114 isolates were successfully biovared,in which 47 cases (41.2%) were biovar 1 ,and 67 cases(58. 8%) were biovar 2;60 isolates were serotyped,in which 41 cases(68.3%) were single serotype,and 19 cases(31.7%) contained multiple serotypes. The most fre-quent serotypes were as follows: serotype 7 (24 cases, 29.63 %);serotype 2 (19 cases, 23.46%);sero-type 14(11 cases,13. 58%);serotype 4(7 cases,8.64%).No serotype 3,5,12,or 13 was detected. The discrepancy between biovar and serotype was found in 16. 7%(10/60) of Uu isolates. Conclusion The main biovar of Uu isolates is biovar 1 in STD out-patients,and serotype 7,2,14 and 4 were dominant. The discrepancy between biovar and serotype exists in some Uu isolates.