CAJ | 학술논문

目的:观察黄酒是否能减轻动脉粥样硬化斑块的形成并探讨其可能机制.方法:48只6周龄LDLR~(-/-)小鼠,以高脂+高蛋氨酸(标准饲料+10%油脂+1.25%胆固醇+1%L-蛋氨酸)喂养诱导形成高同型半胱氨酸血症并致动脉粥样硬化模型,随机分为:黄酒组、红葡萄酒组、酒精组和对照组,每组12只.14周后处死小鼠,检测血脂及血浆同型半胱氨酸;观察胸腹主动脉和主动脉窦部粥样硬化情况;免疫组化测定主动脉窦部MMP-2的表达;明胶酶谱法测定MMP-2的活性.结果:(1)4组间TC、TG、HDL-C水平无显著差别(P＞0.05),黄酒组血浆HCY分别较对照组、酒精组和红葡萄酒组显著下降(P＜0.01).(2)与对照组比较,主动脉粥样硬化斑块面积黄酒组、红葡萄酒组和酒精组分别减少了33.7%、35.9%(P＜0.01)和6.5%(P＞0.05);与酒精组比较,黄酒组和红葡萄酒组主动脉粥样硬化面积差别同样显著(P＜0.01).分别与对照组和酒精组比较,黄酒组和红葡萄酒组主动脉窦部粥样硬化面积减少同样显著差异(P＜0.01),黄酒组和红葡萄酒组间无显著差异(P＞0.05).(3)与对照组比较,MMP-2的表达在黄酒组、红葡萄酒组和酒精组分别减少了26.3%、27.6%(P＜0.01)和5.7%(P＞0.05);MMP-2的活性在黄酒组、红葡萄酒组、酒精组分别减少了31.7%、32.5%(P＜0.01)和6.7%(P＞0.05).结论:黄酒和红葡萄酒均能抑制MMP-2的表达和减轻动脉粥样硬化斑块的形成,这可能是它们对心血管系统保护作用的机制之一.
목적:관찰황주시부능감경동맥죽양경화반괴적형성병탐토기가능궤제.방법:48지6주령LDLR~(-/-)소서,이고지+고단안산(표준사료+10%유지+1.25%담고순+1%L-단안산)위양유도형성고동형반광안산혈증병치동맥죽양경화모형,수궤분위:황주조、홍포도주조、주정조화대조조,매조12지.14주후처사소서,검측혈지급혈장동형반광안산;관찰흉복주동맥화주동맥두부죽양경화정황;면역조화측정주동맥두부MMP-2적표체;명효매보법측정MMP-2적활성.결과:(1)4조간TC、TG、HDL-C수평무현저차별(P＞0.05),황주조혈장HCY분별교대조조、주정조화홍포도주조현저하강(P＜0.01).(2)여대조조비교,주동맥죽양경화반괴면적황주조、홍포도주조화주정조분별감소료33.7%、35.9%(P＜0.01)화6.5%(P＞0.05);여주정조비교,황주조화홍포도주조주동맥죽양경화면적차별동양현저(P＜0.01).분별여대조조화주정조비교,황주조화홍포도주조주동맥두부죽양경화면적감소동양현저차이(P＜0.01),황주조화홍포도주조간무현저차이(P＞0.05).(3)여대조조비교,MMP-2적표체재황주조、홍포도주조화주정조분별감소료26.3%、27.6%(P＜0.01)화5.7%(P＞0.05);MMP-2적활성재황주조、홍포도주조、주정조분별감소료31.7%、32.5%(P＜0.01)화6.7%(P＞0.05).결론:황주화홍포도주균능억제MMP-2적표체화감경동맥죽양경화반괴적형성,저가능시타문대심혈관계통보호작용적궤제지일.
AIM: To study the possibility that yellow wine improves the pathological changes of atherosclerosis in vivo. METHODS: Six weeks old LDL receptor knockout mice (n=48) on a high-fat and L-methionine diet developed plasma hyperhomocysteinemia and atherosclerosis. The animals were randomly divided into yellow wine group, red wine group, ethanol group and control group (n=12 in each group) and were sacrificed after 14 weeks. The levels of plasma lipids and homocysteine in serum were examined. The morphological changes of aorta artery and the atherosclerosis of aorta sinus were observed under microscope. The expression and activation of matrix metalloproteinase-2 (MMP-2) were determined by the method of immunohistochemistry. RESULTS: No significant difference of plasma total cholesterol, triglyceride or high density lipoprotein cholesterol among groups was observed. Plasma homocysteine was significantly decreased in yellow wine group as compared to other three groups (P<0.01). Compared to ethanol and control groups, use of yellow wine and red wine significantly reduced the atherosclerosis lesion area (P<0.01). However, no significant discrepancy between the yellow wine group and red wine group was found. Compared to control group, the expression of MMP-2 in yellow wine group, red wine group and ethanol group decreased by 26.3%, 27.6% (P<0.01) and 5.7% (P>0.05), respectively. The activity of MMP-2 in yellow wine group, red wine group and ethanol group decreased by 31.7%, 32.5% (P<0.01) and 6.7% (P>0.05), respectively. CONCLUSION: Yellow wine and red wine inhibit the expression of MMP-2 and improve the pathologic changes of atherosclerosis, indicating that they have benefic effects on cardiovascular system.