中南工业大学学报(英文版)
中南工業大學學報(英文版)
중남공업대학학보(영문판)
JOURNAL OF CENTRAL SOUTH UNIVERSITY OF TECHNOLOGY(ENGLISH EDITION)
2002年
4期
246-249
,共4页
刘佳佳%赵国玲%王晖%章晓骅
劉佳佳%趙國玲%王暉%章曉驊
류가가%조국령%왕휘%장효화
flos lonicerae%chlorogenic acid%enzymatic treatment%extract process
A new method of extracting chlorogenic acid from flos lonicerae, and treating the materials with enzyme before being extracted by ethanol is developed, and the optimum conditions are also investigated in detail. Three important factors, enzyme dosage, treatment time and treatment temperature are adapted to optimize the extraction process. The experimental results show that the extract yield of flos lonicerae and chlorogenic acid can be obviously increased by the cellulase treatment, 61.5 mg chlorogenic acid is obtained from 1.00 g flos lonicerae at most. The optimal temperature of enzymatic treatment is 40-50 ℃. Compared with the use of single cellulase, the combined treatment of cellulase and pectinase increase the extract yield obviously but fail to improve that of chlorogenic acid.
