短发夹RNA抑制三氧化二砷耐药的白血病K562/AS2细胞MRPI基因表达的实验研究
단발협RNA억제삼양화이신내약적백혈병K562/AS2세포MRPI기인표체적실험연구
Inhibitional effect of small hairpin RNA on expression of MRPI gene in K562/AS2 cell line resistant to As2O3
  • 万方数据
    白血病·淋巴瘤 백혈병·림파류
    JOURNAL OF LEUKEMIA & LYMPHOMA
    2009年 7期 388-391 ,共4页

    苏晓星%卓家才%古庆利%熊文杰%陶小梅%李明%张琼丽%黄瑞宏%刘焕勋%蔡力生%杜新

    소효성%탁가재%고경리%웅문걸%도소매%리명%장경려%황서굉%류환훈%채력생%두신

    RNA%砷剂%抗药性,肿瘤%基因,MRPI%K562细胞 RNA%砷劑%抗藥性,腫瘤%基因,MRPI%K562細胞
    RNA%신제%항약성,종류%기인,MRPI%K562세포
    RNA%Arsenicals%Drug resistance,neoplasm%Genes,MRP1%K562 cells
    目的 研究短发夹RNA(shRNA)对耐三氧化二砷(As2O3)的白血病K562/AS2细胞MRPI基因表达的抑制作用.方法 设计并合成3条针对MRPI基因的shRNA序列,在脂质体的介导下转染K562/AS2细胞;用荧光实时定量PCR分析MRPI mRNA的表达水平;流式细胞术检测MRPI蛋白表达和细胞内柔红霉素蓄积量.结果 经MRPI shRNA作用24 h后,K562/AS2细胞株中MRPImRNA和蛋白表达水平明显下降,最大下调幅度分别为(79.1±0.07)%和(62.48±0.86)%(P<0.05),同时细胞内柔红霉素蓄积量显著增加(P<0.05).结论 MRPI shRNA可抑制As2O3耐药的白血病细胞株K562/AS2细胞MRPl基因的表达.
    목적 연구단발협RNA(shRNA)대내삼양화이신(As2O3)적백혈병K562/AS2세포MRPI기인표체적억제작용.방법 설계병합성3조침대MRPI기인적shRNA서렬,재지질체적개도하전염K562/AS2세포;용형광실시정량PCR분석MRPI mRNA적표체수평;류식세포술검측MRPI단백표체화세포내유홍매소축적량.결과 경MRPI shRNA작용24 h후,K562/AS2세포주중MRPImRNA화단백표체수평명현하강,최대하조폭도분별위(79.1±0.07)%화(62.48±0.86)%(P<0.05),동시세포내유홍매소축적량현저증가(P<0.05).결론 MRPI shRNA가억제As2O3내약적백혈병세포주K562/AS2세포MRPl기인적표체.
    Objective To investigate the inhibitional effect of MRPI-shRNA on expression of MRP1gene in K56.2/AS2 cells resistant to arsenic trioxide. Methods Three pieces of MRPI-shRNA were designed,synthesized and transfected into K562/AS2 cells with lipesome. Expression level of MRPI mRNA were determined by real time fluorescent quantitative PCR. MRPI protein expression and intracellular accumulation of DNR were assayed with flow cytometry. Results After treated with MRPI-shRNA, the expression level of MRPI mRNA and MRPI protein in K562/AS2 cells decreased significantly(79.1±0.07) % and (62.48±0.86) %,respectively (P <0.05). The intracellular accumulation of DNR increased significantly(P < 0.05). Conclusion MRPI-shRNA can down-regulate the expression of MRPI gene in K562/AS2 cell line.
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