CAJ | 학술논문

目的研究HD患儿各段肠管中ATP受体亚型P2Y2的表达情况.初步探讨P2Y2受体表达与HD发生的关系.方法随机选取2000至2008年在我院行手术治疗的HD患儿结肠标本共30例,将HD患儿正常段肠管设为对照组,移行段及痉挛段肠管设为实验组,应用免疫组化及RT-PCR法观察ATP受体P2Y亚型P2Y2在各肠段的表达情况.结果HD患儿正常段肠管的神经节细胞中有大量P2Y2阳性细胞的表达,而在痉挛段肠管中没有P2Y2阳性细胞的表达,移行段组织中可见P2Y2阳性细胞的表达,但其数量明显少于对照组,差别具有统计学意义(P<0.05).RT-PCR结果显示mRNA水平的表达与免疫组化一致.结论 HD患儿痉挛段肠管中ATP受体P2Y2的表达缺失,P2Y2的表达缺失可能与HD的发生有关.
목적 연구HD환인각단장관중ATP수체아형P2Y2적표체정황.초보탐토P2Y2수체표체여HD발생적관계.방법 수궤선취2000지2008년재아원행수술치료적HD환인결장표본공30례,장HD환인정상단장관설위대조조,이행단급경련단장관설위실험조,응용면역조화급RT-PCR법관찰ATP수체P2Y아형P2Y2재각장단적표체정황.결과HD환인정상단장관적신경절세포중유대량P2Y2양성세포적표체,이재경련단장관중몰유P2Y2양성세포적표체,이행단조직중가견P2Y2양성세포적표체,단기수량명현소우대조조,차별구유통계학의의(P<0.05).RT-PCR결과현시mRNA수평적표체여면역조화일치.결론 HD환인경련단장관중ATP수체P2Y2적표체결실,P2Y2적표체결실가능여HD적발생유관.
Objective To investigate the expression of P2Y2 subtype purinergic receptors in normal and spastic segments of colons in patients with Hirschsprung's Disease (HD), and try to reveal the relationship between P2Y2 subtype receptors and pathogenesis of HD. Methods From 2000 to 2008, resected colon specimens of 30 cases with HD were selected for this study. Normal colon segment served as the control group, while the transitional and spastic segments as the experimental group. Im-munohistochemical staining and RT-PCR were applied to detect the expression of P2Y2 subtype recep-tors. Results A large number of P2Y2 positive gangliocytes were observed in the control group,while none were observed in spastic segments, and significantly less P2Y2 positive gangliocytes could be found in the transitional segments (P<0. 05). The results of RT-PCR correlated with the findings of immunohistochemical assay. Conclusions The P2Y2 subtype purinergic receptors are not expressed in the spastic colon segments,which can be related with the pathogenesis of HD.