CAJ | 학술논문

目的研究HIV-1慢性感染者CD8+T淋巴细胞表面CD127(白细胞介素-7受体α链,IL-7Rα)分子的表达及临床意义.方法收集24例HIV-1慢性感染者和12名健康人群的外周血,分离外周血单核细胞(PBMC),用直接免疫荧光染色法标记并用流式细胞仪检测T淋巴细胞表面CD127表达和CD8+T淋巴细胞的自发凋亡,并对CD8+T淋巴细胞CD127的表达与CD4+T淋巴细胞计数、HIV RNA载量以及CD8+T淋巴细胞自发凋亡作相关性分析.组间比较采用Mann-Whitney U检验,Spearman评价组间相关性.结果与健康对照组比较,HIV-1慢性感染组中CD8+T淋巴细胞CD127的表达显著下降,差异具有统计学意义(Z=-4.796,P＜0.01).CD8+T淋巴细胞CD127的表达与患者CD4+T淋巴细胞计数呈显著正相关(r=0.817,P＜0.01),与血浆HIV RNA载量和CD8+T淋巴细胞的自发凋亡呈显著负相关(r=-0.442和-0.688,P＜0.05和＜0.01).结论 HIV-1慢性感染组CD8+T淋巴细胞CD127表达进行性下降可能是导致细胞接受存活信号能力降低和发生凋亡的重要原因,提示IL-7可能是治疗HIV感染的新途径.
목적 연구HIV-1만성감염자CD8+T림파세포표면CD127(백세포개소-7수체α련,IL-7Rα)분자적표체급림상의의.방법 수집24례HIV-1만성감염자화12명건강인군적외주혈,분리외주혈단핵세포(PBMC),용직접면역형광염색법표기병용류식세포의검측T림파세포표면CD127표체화CD8+T림파세포적자발조망,병대CD8+T림파세포CD127적표체여CD4+T림파세포계수、HIV RNA재량이급CD8+T림파세포자발조망작상관성분석.조간비교채용Mann-Whitney U검험,Spearman평개조간상관성.결과 여건강대조조비교,HIV-1만성감염조중CD8+T림파세포CD127적표체현저하강,차이구유통계학의의(Z=-4.796,P＜0.01).CD8+T림파세포CD127적표체여환자CD4+T림파세포계수정현저정상관(r=0.817,P＜0.01),여혈장HIV RNA재량화CD8+T림파세포적자발조망정현저부상관(r=-0.442화-0.688,P＜0.05화＜0.01).결론 HIV-1만성감염조CD8+T림파세포CD127표체진행성하강가능시도치세포접수존활신호능력강저화발생조망적중요원인,제시IL-7가능시치료HIV감염적신도경.
Objective To investigate the expression of CD127 (interleukin-7 receptor α, IL-7Rα)and its association with apoptosis of CD8 + T lymphocytes in patients with chronic HIV-1 infection. Methods The expression of CD127 on T lymphocytes and spontaneous apoptosis of CD8+ T lymphocytes were measured by flow cytometry in peripheral blood from 24 patients with chronic HIV-1 infections and 12 healthy subjects. The associations of CD127 expression with CD4 +T lymphocytes counts, HIV RNA loads and cell apoptosis were analyzed. Mann-Whitney U test was performed to compare between the groups, and Spearman test was used for correlation analysis. Results The expression of CD127 on CD8 + T lymphocytes was significantly down-regulated in HIV-1 infected subjects (Z = -4.796, P ＜ 0. 01 ), which was positively correlated with CD4 + T lymphocytes (r = 0.817, P ＜ 0.01 ) and negatively correlated with HIV RNA load and CD8+T lymphocytes apoptosis (r= -0.442 and -0.688,P＜0.05 and ＜0.01). Conclusion CD127 down-regulation may play an important role in the descended ability of receiving survival signals and ascended apoptosis of CD8 + T lymphocytes during chronic HIV-1 infection, which indicates that IL-7 might be a novel strategy in treatment of HIV infection.