中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2011年
5期
616-617
,共2页
黑色素瘤%Kiss-1基因%基因,肿瘤抑制%细胞凋亡
黑色素瘤%Kiss-1基因%基因,腫瘤抑製%細胞凋亡
흑색소류%Kiss-1기인%기인,종류억제%세포조망
Melanoma Kiss-1%Genes,tumor suppressor,Apoptosis
目的 探讨转染Kiss-1基因对人黑色素瘤细胞A375生长增殖的影响,筛选出有意义的肿瘤治疗的生物学靶标.方法 在人黑色素瘤细胞A375中转染Kiss-1基因,经G418筛选,建立稳定高表达Kiss-1蛋白的细胞系.Western blot方法 检测Kiss-1蛋白以证实转染成功.流式细胞术检测Kiss-1基因对A375生长增殖的影响.结果 稳定转染Kiss-1基因的A375细胞中不仅Kiss-1蛋白稳定表达(1.20±0.21)高于对照组(0.60±0.41),Kiss-1基因转染A375细胞48 h后对其具有凋亡的作用(凋亡率为28.42%)高于对照组(2.12%).结论 外源性Kiss-1基因导入A375细胞后,可诱导A375细胞其凋亡,从而抑制肿瘤细胞增殖.
目的 探討轉染Kiss-1基因對人黑色素瘤細胞A375生長增殖的影響,篩選齣有意義的腫瘤治療的生物學靶標.方法 在人黑色素瘤細胞A375中轉染Kiss-1基因,經G418篩選,建立穩定高錶達Kiss-1蛋白的細胞繫.Western blot方法 檢測Kiss-1蛋白以證實轉染成功.流式細胞術檢測Kiss-1基因對A375生長增殖的影響.結果 穩定轉染Kiss-1基因的A375細胞中不僅Kiss-1蛋白穩定錶達(1.20±0.21)高于對照組(0.60±0.41),Kiss-1基因轉染A375細胞48 h後對其具有凋亡的作用(凋亡率為28.42%)高于對照組(2.12%).結論 外源性Kiss-1基因導入A375細胞後,可誘導A375細胞其凋亡,從而抑製腫瘤細胞增殖.
목적 탐토전염Kiss-1기인대인흑색소류세포A375생장증식적영향,사선출유의의적종류치료적생물학파표.방법 재인흑색소류세포A375중전염Kiss-1기인,경G418사선,건립은정고표체Kiss-1단백적세포계.Western blot방법 검측Kiss-1단백이증실전염성공.류식세포술검측Kiss-1기인대A375생장증식적영향.결과 은정전염Kiss-1기인적A375세포중불부Kiss-1단백은정표체(1.20±0.21)고우대조조(0.60±0.41),Kiss-1기인전염A375세포48 h후대기구유조망적작용(조망솔위28.42%)고우대조조(2.12%).결론 외원성Kiss-1기인도입A375세포후,가유도A375세포기조망,종이억제종류세포증식.
Objective To investigate the effect of Kiss-1 on cell proliferation in human melanoma cancer cell,and screen a target for tumor biotherapy. Methods The eukaryotic expression vectors with Kiss-1 gene were used to transfect A375 cells by liposome-mediated transfection in vitro. The cells were selected by G418. The expression of Kiss-1 gene was confirmed by Western Blotting. FACS was used to detect the ability of cells proliferation and apoptosis. Results There were significantly high expression of Kiss-1 protein in the transfected A375 cells(1.20 ±0.21 vs control 0.60 + 0. 41), whose growth rate was significantly slower than that of control. Flow cytometry showed that apoptosis of A375 cells transfected Kiss-1 were induced(7.23% vs control 4.62%). Conclusion When transfected into A375 cells,exogenous Kiss-1 could cause the cell growth arrest to G1 ,and induce cells apoptosis.
