CAJ | 학술논문

背景单核细胞趋化蛋白-1( MCP-1)是趋化因子家族中的主要成员之一,在肿瘤、炎症和糖尿病视网膜病变(DR)等新生血管性疾病中起着重要的作用,但关于MCP-1在氧诱导视网膜病变(OIR)发生过程中的表达及其作用的研究鲜有报道. 目的观察OIR小鼠视网膜中MCP-1的表达,探讨MCP-1在视网膜血管发育和视网膜新生血管形成过程中的作用. 方法 SPF级C57BL/6J小鼠120只,按随机数字表法随机分为OIR组和正常对照组,每组60只.OIR组将出生后7d的新生小鼠在体积分数75％氧环境下饲养5d,然后返回正常大气环境中；正常对照组小鼠始终置于正常大气环境中饲养.OIR组和正常对照组分别在出生后5、7、12、14、17、21 d随机抽取10只小鼠,摘除右眼球,采用免疫组织化学法检测MCP-1蛋白在小鼠视网膜中的表达情况,采用逆转录-聚合酶链反应(RT-PCR)法检测MCP-1 mRNA在小鼠视网膜中的表达. 结果正常对照组小鼠在出生后的第5天即有MCP-1在视网膜的内核层和节细胞层表达,12d时表达MCP-1的阳性细胞数达到高峰,其他日龄时呈基线表达.OIR组12d时表达MCP-1的阳性细胞数轻度增多,14d时阳性细胞数显著增多,之后迅速下降至正常水平.正常对照组在5d时可检测到MCP-1 mRNA表达,12d时显著上调,之后随小鼠日龄的增加,MCP-1 mRNA表达迅速下降,14、17、21 d表达基本呈基线水平.OIR组12 d时,MCP-1 mRNA较正常对照组下降,在新生血管形成关键期,即14 d时表达显著上调,之后迅速下降至正常水平.OIR组和正常对照组间比较采用完全随机分组的两因素方差分析,F分组=5.230,P=0.028;F日龄=6.898,P=0.001.结论小鼠视网膜发育过程始终伴随着MCP-1的表达,MCP-1的表达上调可能与小鼠视网膜血管发育和OIR模型中视网膜新生血管的形成密切相关.
배경 단핵세포추화단백-1( MCP-1)시추화인자가족중적주요성원지일,재종류、염증화당뇨병시망막병변(DR)등신생혈관성질병중기착중요적작용,단관우MCP-1재양유도시망막병변(OIR)발생과정중적표체급기작용적연구선유보도. 목적 관찰OIR소서시망막중MCP-1적표체,탐토MCP-1재시망막혈관발육화시망막신생혈관형성과정중적작용. 방법 SPF급C57BL/6J소서120지,안수궤수자표법수궤분위OIR조화정상대조조,매조60지.OIR조장출생후7d적신생소서재체적분수75％양배경하사양5d,연후반회정상대기배경중；정상대조조소서시종치우정상대기배경중사양.OIR조화정상대조조분별재출생후5、7、12、14、17、21 d수궤추취10지소서,적제우안구,채용면역조직화학법검측MCP-1단백재소서시망막중적표체정황,채용역전록-취합매련반응(RT-PCR)법검측MCP-1 mRNA재소서시망막중적표체. 결과 정상대조조소서재출생후적제5천즉유MCP-1재시망막적내핵층화절세포층표체,12d시표체MCP-1적양성세포수체도고봉,기타일령시정기선표체.OIR조12d시표체MCP-1적양성세포수경도증다,14d시양성세포수현저증다,지후신속하강지정상수평.정상대조조재5d시가검측도MCP-1 mRNA표체,12d시현저상조,지후수소서일령적증가,MCP-1 mRNA표체신속하강,14、17、21 d표체기본정기선수평.OIR조12 d시,MCP-1 mRNA교정상대조조하강,재신생혈관형성관건기,즉14 d시표체현저상조,지후신속하강지정상수평.OIR조화정상대조조간비교채용완전수궤분조적량인소방차분석,F분조=5.230,P=0.028;F일령=6.898,P=0.001.결론 소서시망막발육과정시종반수착MCP-1적표체,MCP-1적표체상조가능여소서시망막혈관발육화OIR모형중시망막신생혈관적형성밀절상관.
Background Monocyte chemotactic protein-1 (MCP-1)plays an important role in the tumor,inflammation,diabetic retinopathy and other neovascular disease,but the expression and the role of MCP-1 in the oxygen induced retinopathy(OIR) model have rarely been reported. Objective This study was to investigate the expression of MCP-1 in the retina development of newborn mouse and in mouse models with OIR. Methods C57BL/6J newborn mice were divided into two groups and 60 mice in each group.Mice in OIR group were exposed to 75％ oxygen for 5 days and then to room air.All mice in normal control group exposed to room air only.Ten mice in each group were randomly chosen and sacrificed at postnatal 5,7,12,14,17,21 days.The expression of MCP-1 in mouse retina was detected with the method of immunohistoehemistry and reverse transcription polymerase chain reaction(RT-PCR). Results MCP-1 positive cells were seen in normal mouse retina.Up-regulation of MCP-1 positive cells was detected both in 12 days in normal control group and in 14 days in OIR group.MCP-1 mRNA was detected in mouse retina at 5 days,and a transient up-regulation of MCP-1 mRNA was observed in 12 days in normal control group.MCP-1 mRNA in OIR group significantly increased in 14 days in comparison with the normal control group( P =0.028,P =0.001 ). Conclusions Expression of MCP-1 is detectable in whole retinal development procession of mice.A transient up-regulation of MCP-1 expression is detected in the critical period of retinal vascular development in mice models with OIR,which is closely related to the retinal vascular development and progression of retinal new vessels.