CAJ | 학술논문

目的探讨外周血吲哚胺2,3双加氧酶(IDO)基因表达对大鼠肝移植急性排斥反应(AR)的诊断价值.方法建立大鼠原位肝移植模型,分为4组:A组:同基因移植组(Wistar-Wistar,n=32);B组:异基因移植组(SD-Wistar,n=32);C组:异基因移植+长期环孢素A组(CsA,n=32);D组:异基因移植+短期CsA组(用药剂量同C组,第3天起停药,n=32).应用实时聚合酶链反应(Real-time PCR)方法分别检测术后第0、1、2、3、4、5、7、9天外周血IDO mRNA值,同时检测各时间点血清谷草转氨酶(AST)、总胆红素(T-BIL)、碱性磷酸酶(ALP)水平,并取肝脏病理切片.结果 A组外周血IDO mRNA呈持续低水平,AST、T-BIL、ALP逐渐降至正常,病理无排斥反应.C组检测结果与A组相似.B组IDO mRNA显著上升为术后第2天(P<0.05),AST、T-BIL、ALP值显著上升为术后4d(P<0.01),病理切片判断轻度排斥为术后5d(χ2=4.8,P<0.05).D组IDO mRNA显著上升为术后第4天(P<0.05),AST、T-BIL、ALP值显著上升为术后5d(P<0.01),病理切片判断轻度排斥为术后7d(χ2=4.8,P<0.05).结论外周血IDO mRNA检测可较病理检查更早诊断大鼠肝移植AR的发生,且方法简单、安全.
목적 탐토외주혈신타알2,3쌍가양매(IDO)기인표체대대서간이식급성배척반응(AR)적진단개치.방법 건립대서원위간이식모형,분위4조:A조:동기인이식조(Wistar-Wistar,n=32);B조:이기인이식조(SD-Wistar,n=32);C조:이기인이식+장기배포소A조(CsA,n=32);D조:이기인이식+단기CsA조(용약제량동C조,제3천기정약,n=32).응용실시취합매련반응(Real-time PCR)방법 분별검측술후제0、1、2、3、4、5、7、9천외주혈IDO mRNA치,동시검측각시간점혈청곡초전안매(AST)、총담홍소(T-BIL)、감성린산매(ALP)수평,병취간장병리절편.결과 A조외주혈IDO mRNA정지속저수평,AST、T-BIL、ALP축점강지정상,병리무배척반응.C조검측결과 여A조상사.B조IDO mRNA현저상승위술후제2천(P<0.05),AST、T-BIL、ALP치현저상승위술후4d(P<0.01),병리절편판단경도배척위술후5d(χ2=4.8,P<0.05).D조IDO mRNA현저상승위술후제4천(P<0.05),AST、T-BIL、ALP치현저상승위술후5d(P<0.01),병리절편판단경도배척위술후7d(χ2=4.8,P<0.05).결론 외주혈IDO mRNA검측가교병리검사경조진단대서간이식AR적발생,차방법 간단、안전.
Objective To study the diagnostic value of indoleamine 2, 3-dioxygenase (IDO) gene expression in acute liver rejection in rat orthotopic liver transplantation model. Methods The rat orthotopic liver transplantation models were divided into four groups: group A, isograft transplantation group (Wistar to Wistar); group B, allograft transplantation (SD to Wistar); group C, allograft transplantation and cyclosporine; Group D, allograft transplantation and cyclosporine (the drug was withdrawn on the 3rd day after the operation). The samples (peripheral blood and liver tissue) were obtained on the operation day, 1st, 2nd, 3rd, 4th, 5th, 7th and 9th day post-operation. Luorescent quantitative polymerase chain reaction (PCR), pathological study and serum test were performed on each sample. Results In groups A and C, the IDO mRNA was expressed at a low level, aspartate aminotransferase (AST), total bilirubin (T-BIL) and alkaline phosphatase (ALP) were decreased to the normal level, and the pathological test found no acute rejection (AR). In group B, the IDO mRNA was dramatically increased on the day 2 (P<0.05), AST, T-BIL and ALP rose dramatically on the day 4 (P<0.05), and mild AR was detected on the day 5 (χ2=4.8,P<0.05). In group D, the IDO mRNA was dramatically increased on the day 4 (P<0.05), AST, T-BIL and ALP rose dramatically on the day 5 (P<0.01), and mild AR was detected on the day 7 (χ2=4.8,P<0.05). Conclusion Comparing with pathological study, detecting IDO mRNA of peripheral blood can diagnose AR of rat at earlier stage and the technique is safe and simple.