中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
2期
200-202
,共3页
范子扬%宋振顺%李煜环%滕洪飞%张福琴
範子颺%宋振順%李煜環%滕洪飛%張福琴
범자양%송진순%리욱배%등홍비%장복금
骨髓间充质干细胞%基质细胞衍生因子-1%胰岛%再生
骨髓間充質榦細胞%基質細胞衍生因子-1%胰島%再生
골수간충질간세포%기질세포연생인자-1%이도%재생
Bone marrow mesenchymal stem cells%SDF-1%Islet%Regeneration
目的 观察同种异体骨髓间充质干细胞(MSCs)移植入受体后,基质细胞衍生因子(SDF)-1/CXCR4轴在促进残存胰岛及其周围新生血管增殖中的作用.方法 对大鼠MSCs进行体外培养、鉴定.链脲佐菌素(STZ)诱导的糖尿病大鼠随机分为A组(MSCs移植组)、B组(MSCs移植+SDF-I/CXCR4轴阻断剂AMD组)和C组(糖尿病对照组),另设D组(正常大鼠对照组).移植MSCs后第30天取出各组大鼠胰腺和血清,胰腺组织采用苏木素-伊红(HE)染色和免疫组织化学法观察CD31、增殖细胞核抗原(PCNA)、胰腺干细胞标志物(PDX)-1在胰腺组织的表达水平.血糖仪检测血糖水平、放免法检测胰岛素水平、酶联免疫吸附试验(ELISA)检测SDF-1水平.结果 (1)A组残存胰岛周围可见新生血管,CD31、PCNA、PDX-1染色阳性率分别为(71.2±5.3)%、(76.5±4.5)%、(69.8±6.7)%;B组残存胰岛周围基本未见新生血管,CD31、PCNA、PDX-1染色阳性率分别为(7.4±2.1)%、(5.5±3.7)%、(8.8±2.9)%,两组比较差异有统计学意义(P＜0.05).(2)移植后第25天,A组血糖浓度基本正常,低于B组和C组,而胰岛素水平明显高于B组和C组(P＜0.05).(3)A组与B组血清SDF-1水平差异无统计学意义(P＞0.05),但都明显高于C组(P＜0.05).结论 MSCs促进胰岛再生和新生血管形成,AMD3100能抑制MSCs的作用,进而提示SDF-1/CXCR4轴在胰岛再生和血管形成中具有重要作用.
目的 觀察同種異體骨髓間充質榦細胞(MSCs)移植入受體後,基質細胞衍生因子(SDF)-1/CXCR4軸在促進殘存胰島及其週圍新生血管增殖中的作用.方法 對大鼠MSCs進行體外培養、鑒定.鏈脲佐菌素(STZ)誘導的糖尿病大鼠隨機分為A組(MSCs移植組)、B組(MSCs移植+SDF-I/CXCR4軸阻斷劑AMD組)和C組(糖尿病對照組),另設D組(正常大鼠對照組).移植MSCs後第30天取齣各組大鼠胰腺和血清,胰腺組織採用囌木素-伊紅(HE)染色和免疫組織化學法觀察CD31、增殖細胞覈抗原(PCNA)、胰腺榦細胞標誌物(PDX)-1在胰腺組織的錶達水平.血糖儀檢測血糖水平、放免法檢測胰島素水平、酶聯免疫吸附試驗(ELISA)檢測SDF-1水平.結果 (1)A組殘存胰島週圍可見新生血管,CD31、PCNA、PDX-1染色暘性率分彆為(71.2±5.3)%、(76.5±4.5)%、(69.8±6.7)%;B組殘存胰島週圍基本未見新生血管,CD31、PCNA、PDX-1染色暘性率分彆為(7.4±2.1)%、(5.5±3.7)%、(8.8±2.9)%,兩組比較差異有統計學意義(P＜0.05).(2)移植後第25天,A組血糖濃度基本正常,低于B組和C組,而胰島素水平明顯高于B組和C組(P＜0.05).(3)A組與B組血清SDF-1水平差異無統計學意義(P＞0.05),但都明顯高于C組(P＜0.05).結論 MSCs促進胰島再生和新生血管形成,AMD3100能抑製MSCs的作用,進而提示SDF-1/CXCR4軸在胰島再生和血管形成中具有重要作用.
목적 관찰동충이체골수간충질간세포(MSCs)이식입수체후,기질세포연생인자(SDF)-1/CXCR4축재촉진잔존이도급기주위신생혈관증식중적작용.방법 대대서MSCs진행체외배양、감정.련뇨좌균소(STZ)유도적당뇨병대서수궤분위A조(MSCs이식조)、B조(MSCs이식+SDF-I/CXCR4축조단제AMD조)화C조(당뇨병대조조),령설D조(정상대서대조조).이식MSCs후제30천취출각조대서이선화혈청,이선조직채용소목소-이홍(HE)염색화면역조직화학법관찰CD31、증식세포핵항원(PCNA)、이선간세포표지물(PDX)-1재이선조직적표체수평.혈당의검측혈당수평、방면법검측이도소수평、매련면역흡부시험(ELISA)검측SDF-1수평.결과 (1)A조잔존이도주위가견신생혈관,CD31、PCNA、PDX-1염색양성솔분별위(71.2±5.3)%、(76.5±4.5)%、(69.8±6.7)%;B조잔존이도주위기본미견신생혈관,CD31、PCNA、PDX-1염색양성솔분별위(7.4±2.1)%、(5.5±3.7)%、(8.8±2.9)%,량조비교차이유통계학의의(P＜0.05).(2)이식후제25천,A조혈당농도기본정상,저우B조화C조,이이도소수평명현고우B조화C조(P＜0.05).(3)A조여B조혈청SDF-1수평차이무통계학의의(P＞0.05),단도명현고우C조(P＜0.05).결론 MSCs촉진이도재생화신생혈관형성,AMD3100능억제MSCs적작용,진이제시SDF-1/CXCR4축재이도재생화혈관형성중구유중요작용.
Objective To investigate the role of stromal cell derived factor-1 (SDF-1)/CXCR4axis in recipients' remnant islets regeneration and neovascularization after the transplantation of allogeneic bone marrow mesenchymal stem cells (MSCs). Methods MSCs were isolated from SD rats, cultured in vitro and identified by testing the phenotypes with flow cytometry ( FCM ). The diabetic rats induced by streptozotozin were randomly divided into group A ( MSCs transplant group), group B ( MSCs transplant +AMD group) and group C ( DM control group). Group D serve as the normal control. The pancreata were removed and blood serum was retrieved from each group simultaneously at the 13th day after MSCs transplant. The expression of CD31, proliferating cell nuclear antigen (PCNA) and PDX-1 in each group of pancreas tissue was detected by using immunohistochemistry, and the morphological changes in the isletswere observed by Hematoxylin and Eosin (HE) staining. Serum glucose and insulin levels were determined by blood glucose monitor, radioimmunoscintigraphy, and SDF-1 in serum was by enzyme linked immunosorbent assay (ELISA). Results Neovascularization was observed in the remnant islets of the recipient pancreatic tissue and CD31 -positive cells (71.2 ± 5.3 ) %, PCNA-positive cells ( 76. 5 ± 4. 5 ) %, PDX-1-positive cells (69. 8 ±6. 7)% were highly expressed in group A. As compared with group A, seldom-positive cells[CD31 (7.4±2. 1)%, PCNA (5.5 ±3.7)% and PDX-1 (8.8 ±2.9)%]and rarely neovascularization were observed in group B (P ＜0. 05 ). Serum glucose level in group A was lower than that in group B and group C, but serum insulin level in group A was significantly higher than that in group B and group C (P ＜ 0. 05 ). There was no significant difference between group A and group B in serum SDF-1level ( P ＞ 0. 05 ), but that was higher in groups A and B than in group C ( P ＜ 0. 05 ). Conclusion Obviously, MSCs promote recipient neovascularization surrounding the islets, which enhances the proliferation and regeneration of remnant islets. AMD 3100 has the function of intervening SDF-1/CXCR4 axis,which inhibits the effect of MSCs on promoting islets regeneration. It is suggested that SDF-1/CXCR4 axis may play an important role in vascularization and islets regeneration.
