中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
3期
178-181
,共4页
田林强%郭风劲%虞姬哲%陈安民
田林彊%郭風勁%虞姬哲%陳安民
전림강%곽풍경%우희철%진안민
压应力%成骨细胞%骨桥蛋白%骨钙素%碱性磷酸酶
壓應力%成骨細胞%骨橋蛋白%骨鈣素%堿性燐痠酶
압응력%성골세포%골교단백%골개소%감성린산매
Compressive stress%Osteoblasts%Osteopontin%Osteocalcin%Alkaline phosphatase
目的 观察不同强度压应力刺激对大鼠成骨细胞骨桥蛋白(OPN)、核结合因子α1(Runx2)、骨钙素(OC)、Osterix、碱性磷酸酶(ALP)及骨形态发生蛋白2(BMP-2) mRNA表达的影响.方法 采用酶消化法从胎鼠颅骨中提取成骨细胞,经体外扩增后采用ALP染色及茜素红染色法对纯化后的细胞进行鉴定；将所培养成骨细胞按随机数字表法分为对照组、20 mmHg实验组、50 mmHg实验组及100 mmHg实验组,分别置于压力加载装置中给予不同强度(压应力强度分别为0、20、50和100 mmHg)压应力刺激.于压应力刺激24h后采用实时定量PCR技术检测各组细胞OPN、Runx2、OC、Osterix、ALP及BMP-2 mRNA表达情况.结果 成骨细胞在20 mmHg压应力刺激下,其OPN、Runx2、OC、BMP-2及ALP mRNA表达均较对照组明显升高(P＜0.05),Osterix mRNA表达亦较对照组增强,但组间差异无统计学意义(P＞0.05)；在50 mmHg压应力刺激下,成骨细胞OPN、Runx2、OC、Osterix、BMP-2和ALP mRNA表达亦较对照组进一步增高(P＜0.05)；在100 mmHg压应力刺激下,成骨细胞OPN、Runx2、OC、Osterix、BMP-2及ALP mRNA表达虽较对照组有所增强,但此时组间差异均无统计学意义(P＞0.05).结论 一定强度范围的压应力刺激能够促进成骨细胞OPN、Runx2、OC、Osterix、ALP及BMP-2 mRNA表达,这可能是压应力刺激促进骨折愈合的重要机制之一.
目的 觀察不同彊度壓應力刺激對大鼠成骨細胞骨橋蛋白(OPN)、覈結閤因子α1(Runx2)、骨鈣素(OC)、Osterix、堿性燐痠酶(ALP)及骨形態髮生蛋白2(BMP-2) mRNA錶達的影響.方法 採用酶消化法從胎鼠顱骨中提取成骨細胞,經體外擴增後採用ALP染色及茜素紅染色法對純化後的細胞進行鑒定；將所培養成骨細胞按隨機數字錶法分為對照組、20 mmHg實驗組、50 mmHg實驗組及100 mmHg實驗組,分彆置于壓力加載裝置中給予不同彊度(壓應力彊度分彆為0、20、50和100 mmHg)壓應力刺激.于壓應力刺激24h後採用實時定量PCR技術檢測各組細胞OPN、Runx2、OC、Osterix、ALP及BMP-2 mRNA錶達情況.結果 成骨細胞在20 mmHg壓應力刺激下,其OPN、Runx2、OC、BMP-2及ALP mRNA錶達均較對照組明顯升高(P＜0.05),Osterix mRNA錶達亦較對照組增彊,但組間差異無統計學意義(P＞0.05)；在50 mmHg壓應力刺激下,成骨細胞OPN、Runx2、OC、Osterix、BMP-2和ALP mRNA錶達亦較對照組進一步增高(P＜0.05)；在100 mmHg壓應力刺激下,成骨細胞OPN、Runx2、OC、Osterix、BMP-2及ALP mRNA錶達雖較對照組有所增彊,但此時組間差異均無統計學意義(P＞0.05).結論 一定彊度範圍的壓應力刺激能夠促進成骨細胞OPN、Runx2、OC、Osterix、ALP及BMP-2 mRNA錶達,這可能是壓應力刺激促進骨摺愈閤的重要機製之一.
목적 관찰불동강도압응력자격대대서성골세포골교단백(OPN)、핵결합인자α1(Runx2)、골개소(OC)、Osterix、감성린산매(ALP)급골형태발생단백2(BMP-2) mRNA표체적영향.방법 채용매소화법종태서로골중제취성골세포,경체외확증후채용ALP염색급천소홍염색법대순화후적세포진행감정；장소배양성골세포안수궤수자표법분위대조조、20 mmHg실험조、50 mmHg실험조급100 mmHg실험조,분별치우압력가재장치중급여불동강도(압응력강도분별위0、20、50화100 mmHg)압응력자격.우압응력자격24h후채용실시정량PCR기술검측각조세포OPN、Runx2、OC、Osterix、ALP급BMP-2 mRNA표체정황.결과 성골세포재20 mmHg압응력자격하,기OPN、Runx2、OC、BMP-2급ALP mRNA표체균교대조조명현승고(P＜0.05),Osterix mRNA표체역교대조조증강,단조간차이무통계학의의(P＞0.05)；재50 mmHg압응력자격하,성골세포OPN、Runx2、OC、Osterix、BMP-2화ALP mRNA표체역교대조조진일보증고(P＜0.05)；재100 mmHg압응력자격하,성골세포OPN、Runx2、OC、Osterix、BMP-2급ALP mRNA표체수교대조조유소증강,단차시조간차이균무통계학의의(P＞0.05).결론 일정강도범위적압응력자격능구촉진성골세포OPN、Runx2、OC、Osterix、ALP급BMP-2 mRNA표체,저가능시압응력자격촉진골절유합적중요궤제지일.
Objective To study the impacts of dynamic compressive stress on the mRNA expression of osteopontin ( OPN ),runt related gene 2 ( Runx2 ),osteocalcin ( OC ),osterix,alkaline phosphatase (ALP) and bone morphogenetic protein 2 (BMP-2) in the osteoblasts of Sprague-Dawley (SD) rats. Methods Osteoblasts extracted from skull periosteum tissue of neonatal SD rats were digested using trypsin and collagenase （Ⅰ）,then were subcultured and amplified in vitro.ALP staining and alizarin red staining were performed to identify the purified cells.The cells were treated with compressive stress at 20,50 or 100 mmHg for 24 h.The expression levels of OPN,Runx-2,OC,osterix,ALP and BMP-2 were measured and quantitatively analysed using a real-time quantitative polymerase chain reaction. Results Under 20 mmHg of dynamic compressive stress the expression levels of OPN,Runx2,OC,osterix,ALP and BMP-2 all were elevated compared with the control group.The peak expression oecured under 50 mmHg pressure. The expression levels did not change significantly compared with the control group under 100 mmHg pressure. Conclusions Moderate dynamic compressive stress can promote the expression of OPN,Runx-2,OC,osterix,ALP and BMP-2 mRNA in osteoblasts,which might be an important mechanism for promoting the union of fractures.
