CAJ | 학술논문

目的研究胃癌组织中PTEN和p27蛋白的表达及二者间的相关性.方法应用免疫组织化学方法检测49例胃癌组织、49例癌旁组织和13例正常胃黏膜中PTEN和p27蛋白的表达情况.结果胃癌组织中PTEN和p27蛋白的阳性表达率分别为61.22％(30/49)和57.14％(28/49),较癌旁组[91.84％(45/49)、93.88％(46/49)]和正常组织[100％(13/13)、100％(13/13)]明显降低(均P＜0.05).两者在胃癌中的表达有相关性(r=0.580,P=0.000),且均与肿瘤的分化程度、淋巴结转移和PTNM分期有关.结论 PTEN和p27基因的异常改变可能参与胃黏膜细胞的恶性转化过程,且两者之间存在相关性.联合检测两者表达水平可作为评价胃癌病理生物学行为的客观指标之一.
목적 연구위암조직중PTEN화p27단백적표체급이자간적상관성.방법 응용면역조직화학방법검측49례위암조직、49례암방조직화13례정상위점막중PTEN화p27단백적표체정황.결과 위암조직중PTEN화p27단백적양성표체솔분별위61.22％(30/49)화57.14％(28/49),교암방조[91.84％(45/49)、93.88％(46/49)]화정상조직[100％(13/13)、100％(13/13)]명현강저(균P＜0.05).량자재위암중적표체유상관성(r=0.580,P=0.000),차균여종류적분화정도、림파결전이화PTNM분기유관.결론 PTEN화p27기인적이상개변가능삼여위점막세포적악성전화과정,차량자지간존재상관성.연합검측량자표체수평가작위평개위암병리생물학행위적객관지표지일.
Objective To investigate the correlation between PTEN and p27 expression in gastric carcinoma. Methods Immunohistochemistry was applied to examine the expression of PTEN and p27 in 49 cases of human gastric carcinoma,49 cases of tumor-side ward mucosa and 13 cases of normal gastric mucosa.Results Positive expressive rates of PTEN and p27 protein in colorectal carcinoma were 61.22 ％ (30/49)and 57.14 ％ (28/49),which were lower than those in tumor-side ward mucosa [91.84 ％(45/49) and 93.88％(46/49)] and normal colorectal mucosa [100 ％(13/13) and 100 ％(13/13)](P＜ 0.05). The expression of PTEN and p27 in gastric carcinoma correlated with each other (r=0.580,P=0.005),and which were associated with degree of differentiation of carcinoma,PTNM stage and lymph node metastasis of the tumors.Conclusion The abnormal expression of PTEN and p27 participate the malignant conversion process of the gastric mucosa cell and the expression levels of PTEN and p27 protein may be used to evaluate the pathologic biology behavior of gastric carcinoma.