CAJ | 학술논문

水稻(Oryza sativa L.)雄性不育系珍汕97A、保持系珍汕97B和恢复系测64三系小孢子发生过程的研究表明:恢复系测64小孢子母细胞细胞质浓,有明显的微管荧光围绕着细胞核.小孢子母细胞经两次减数分裂形成四分体.四分体和小孢子的微管从细胞核表面向胞质周缘延伸,形成放射性排列格局,花粉发育正常.细胞质中有少量点状微管荧光.保持系珍汕97B小孢子发生过程的细胞形态和微管结构与恢复系测64相似,但细胞质中的点状微管荧光多一些.雄性不育系珍汕97A小孢子发生早期,小孢子母细胞内出现液泡,核中染色质凝集,微管荧光很弱,没有清晰的微管丝结构,细胞质中有许多点状微管荧光等不正常现象.小孢子母细胞经过减数分裂形成的四分体也没有清晰的丝状微管结构.随后,所有的小孢子迅速败育.雄性不育系珍汕97A在小孢子母细胞发生的很早时期,微管结构就明显不正常.
수도(Oryza sativa L.)웅성불육계진산97A、보지계진산97B화회복계측64삼계소포자발생과정적연구표명:회복계측64소포자모세포세포질농,유명현적미관형광위요착세포핵.소포자모세포경량차감수분렬형성사분체.사분체화소포자적미관종세포핵표면향포질주연연신,형성방사성배렬격국,화분발육정상.세포질중유소량점상미관형광.보지계진산97B소포자발생과정적세포형태화미관결구여회복계측64상사,단세포질중적점상미관형광다일사.웅성불육계진산97A소포자발생조기,소포자모세포내출현액포,핵중염색질응집,미관형광흔약,몰유청석적미관사결구,세포질중유허다점상미관형광등불정상현상.소포자모세포경과감수분렬형성적사분체야몰유청석적사상미관결구.수후,소유적소포자신속패육.웅성불육계진산97A재소포자모세포발생적흔조시기,미관결구취명현불정상.
Histological changes that occur during microsporogenesis are documented in a gene-cytoplasmic male sterile rice (Oryza sativa L.) line, Zhen Shan 97A, its maintainer line, Zhen Shan 97B, and the restorer line, Ce64 of a 3-line hybrid rice production system. In the restorer line, Ce64, the developing microsporocytes have dense cytoplasm and a distinct set of circumferential microtubules around the nucleus. Successive cytokinesis results in the formation of tetrads. The microtubules within the cells of tetrads and microspores radiate from the surface of the nucleus towards the outer edge of the cytoplasm. Subsequent pollen development is normal. During the course of microspore formation tubulin speckles can be found in the cytoplasm. The general pattern of development and microtubule organization in the maintainer line, Zhen Shan 97B, is similar to Ce64, except that a few more tubulin speckles appear during microspore formation. In the case of the male sterile line, Zhen Shan 97A, a number of abnormalities can be discerned during early microsporogenesis. These include vacuoles forming within the developing microsporocyte and faintly stained microtubules with no defined distribution pattern. Prominent tubulin speckles are common within the cytoplasm. For those microsporocytes that undergo meiosis, no defined organizational patterns of microtubules can be found within the tetrad. All microspores abort soon after. Abnormalities and defects in microtubule organization observed in Zhen Shan 97A showed that complex interactions between the cytoplasm and the nucleus began at very early stage of microsporocyte development.