CAJ | 학술논문

利用RT-PCR和RACE在麝香百合(Lilium longiflorum Thunb.)花粉管中克隆到1,4-β-葡聚糖内切酶(Endo-1,4-β-glucanase,EGase)的全长cDNA序列(LlpCel1).序列分析结果表明:该基因编码一个含有490个氨基酸的球状蛋白,并在N端有一个由21个氨基酸构成的信号肽序列.序列比对结果显示,LlpCel1和植物分泌型1,4-β-葡聚糖内切酶高度同源(约50%),不含有跨膜结构域和纤维素结合域(CBD).Northern杂交结果显示,该基因的转录本仅在花粉粒、萌发中的花粉和花粉管生长过程中表达,表达量基本相同.而在百合植株其他组织中均未见表达.这种葡聚糖内切酶的高度特异表达说明LlpCel1对花粉萌发和花粉管伸长起着重要作用.
이용RT-PCR화RACE재사향백합(Lilium longiflorum Thunb.)화분관중극륭도1,4-β-포취당내절매(Endo-1,4-β-glucanase,EGase)적전장cDNA서렬(LlpCel1).서렬분석결과표명:해기인편마일개함유490개안기산적구상단백,병재N단유일개유21개안기산구성적신호태서렬.서렬비대결과현시,LlpCel1화식물분비형1,4-β-포취당내절매고도동원(약50%),불함유과막결구역화섬유소결합역(CBD).Northern잡교결과현시,해기인적전록본부재화분립、맹발중적화분화화분관생장과정중표체,표체량기본상동.이재백합식주기타조직중균미견표체.저충포취당내절매적고도특이표체설명LlpCel1대화분맹발화화분관신장기착중요작용.
A full-length cDNA (LlpCel1) encoding an endo-1,4-β-glucanase (EGase) was isolated from pollen tubes of Liliurm longiflorum Thunb. by RT-PCR and RACE. The deduced protein, which is predicted to be a compact globular protein, is of 490 amino acids, including a putative 21 amino acid-signal peptide.LlpCel1 shares high level of homology (about 50%) with plant secreted EGases, which bear apparently neither trans-membrane domain, nor cellulose-binding domain (CBD). LlpCel1 belongs to glycosyl hydrolase family 9. LlpCel1 transcripts were detected in pollen grains, germinating pollen and elongating pollen tubes with a similar level, whereas LlpCel1 transcripts were not detectable in all other lily tissues examined. The specific expression pattern suggests that LlpCel1 is confined to lily pollen germination and pollen tube elongation.