CAJ | 학술논문

目的建立使用反相高效液相法测定天葵子药材中苯骈呋喃酮型内酯格列风内酯含量的方法.方法使用迪马Kromasil ODS柱,流动相为甲醇-水(含0.01 %三氟醋酸,0.05 %四氢呋喃) (3∶97),检测波长为 260 nm.结果在0.004 - 0.4 mg·mL-1 的范围内,峰面积与浓度线性良好,相关系数为r = 1.000 00;平均回收率为 98.77 %; RSD = 2.77 %.结论本文对天葵子药材进行了质量控制方面的研究,并首次报道了格列风内酯的含量测定方法.本法快速、准确、灵敏度高,可以作为天葵子药材中格列风内酯含量测定的方法.
목적 건립사용반상고효액상법측정천규자약재중분병부남동형내지격렬풍내지함량적방법.방법 사용적마Kromasil ODS주,류동상위갑순-수(함0.01 %삼불작산,0.05 %사경부남) (3∶97),검측파장위 260 nm.결과 재0.004 - 0.4 mg·mL-1 적범위내,봉면적여농도선성량호,상관계수위r = 1.000 00;평균회수솔위 98.77 %; RSD = 2.77 %.결론 본문대천규자약재진행료질량공제방면적연구,병수차보도료격렬풍내지적함량측정방법.본법쾌속、준학、령민도고,가이작위천규자약재중격렬풍내지함량측정적방법.
Aim To establish the method for determination of benzofuranones derivative, griffon-ilide, in the roots of Semiaquilegia adoxoides (DC.) Makino by RP- HPLC. Methods Kromasil C18 column was used with a mobile phase of methanol-water (containing 0.01 % trifluoroacetic acid and 0.05 % tetrahydrofuran) (3∶97). The detecting wavelength was 260 nm. Results The calibration curve was linear over the range of 0.004 - 0.4 mg·mL-1 with the correlation coefficient of 1.00 000. The average recovery was 98.77 % with RSD 2.77 %. Determinations of ten samples from different areas showed that the contents of griffonilide in the roots of S.adoxoides are 0.17-0.49 mg·g-1. Conclusion The method provides an accurate and sensitive way to detect griffonilide in the roots of S.adoxoides.