CAJ | 학술논문

采用原生质体融合技术,构建了脱色工程菌H-1,它可同时对结构差异大的还原大红R和蓝色盐VB染料进行脱色.确定了工程菌H-1的较佳脱色条件为厌氧、30℃、pH=7、接种量为3 g·L~(-1).对色度为417倍、631倍、812倍、1093倍的上述两种染料培养基分别脱色12 h,其中H-1对色度为417倍的蓝色盐VB的脱色率为85.5%,对还原大红R的脱色率为90.1%;对色度为1093倍的蓝色盐VB的脱色率为81.3 %,对还原大红R脱色率为86.5%.同时用单株原始菌株与混合菌对相同浓度的染料培养基脱色,对各菌的脱色结果进行了理论分析.工程菌扩大了脱色种类,提高了降解速率,具有广阔的应用前景.
채용원생질체융합기술,구건료탈색공정균H-1,타가동시대결구차이대적환원대홍R화람색염VB염료진행탈색.학정료공정균H-1적교가탈색조건위염양、30℃、pH=7、접충량위3 g·L~(-1).대색도위417배、631배、812배、1093배적상술량충염료배양기분별탈색12 h,기중H-1대색도위417배적람색염VB적탈색솔위85.5%,대환원대홍R적탈색솔위90.1%;대색도위1093배적람색염VB적탈색솔위81.3 %,대환원대홍R탈색솔위86.5%.동시용단주원시균주여혼합균대상동농도적염료배양기탈색,대각균적탈색결과진행료이론분석.공정균확대료탈색충류,제고료강해속솔,구유엄활적응용전경.
Engineering strain H-1 was constructed by protoplast fusion technology, and it can simultaneously decolorize dyes with great structure difference, such as the vat scarlet R and blue salt VB. The optimum decolorization conditions of using strain H-1 were determined by experiments and they are the temperature of 30℃, pH=7.0, inoculation of 3 g?L-1 and under anaerobic condition. The experiments for the decolorization of above two dye culture media with selected chrominance times of 417, 631, 812 and 1093 respectively were carried out each for 12 h. The results show that the decolorization ratio of blue salt VB and vat scarlet R with chrominance times of 417 reach 85.5% and 90.1%, respectively, and those with chrominance times of 1093 reach 81.3% and 86.5%, respectively. The decolorizations of using original single strains and mixed strains for the dye media with the same concentration were studied as well, and the theoretical analysis of the decolorization results of every strain used were given out. Comparing with the original strain and mixed strains, gene engineering strain H-1 can decolorize more dye species and has higher decolorization ratio, those will bring the engineering strain H-1 a broad prospect of application.