江苏农业学报
江囌農業學報
강소농업학보
JIANGSU JOURNAL OF AGRICULTURAL SCIENCES
2009年
6期
1243-1246
,共4页
吴觐宇%傅玉凡%张华玲%张启堂%GEORGE CRAIG Y
吳覲宇%傅玉凡%張華玲%張啟堂%GEORGE CRAIG Y
오근우%부옥범%장화령%장계당%GEORGE CRAIG Y
甘薯%ISSR%种质资源%亲缘关系
甘藷%ISSR%種質資源%親緣關繫
감서%ISSR%충질자원%친연관계
sweetpotato%ISSR marker%genetic diversity
对25份美国甘薯[Ipomoea batatas (L.) Lam.]品系及另外作为对照的47份甘薯种质资源进行ISSR分析,利用NTSYS软件分析遗传相似系数,采用UPGMA方法进行聚类,构建亲缘关系系统图.结果显示:10个引物共扩增出78个条带,其中有73条呈现多态性,多态位点比例高达93.6%;25份美国甘薯育种材料与对照种质资源的平均遗传相似性系数是0.74.表明美国甘薯育种材料遗传多样性较高,可将其中的6-3-1、6-8-7等品系应用于国内甘薯育种中,以扩大遗传背景.
對25份美國甘藷[Ipomoea batatas (L.) Lam.]品繫及另外作為對照的47份甘藷種質資源進行ISSR分析,利用NTSYS軟件分析遺傳相似繫數,採用UPGMA方法進行聚類,構建親緣關繫繫統圖.結果顯示:10箇引物共擴增齣78箇條帶,其中有73條呈現多態性,多態位點比例高達93.6%;25份美國甘藷育種材料與對照種質資源的平均遺傳相似性繫數是0.74.錶明美國甘藷育種材料遺傳多樣性較高,可將其中的6-3-1、6-8-7等品繫應用于國內甘藷育種中,以擴大遺傳揹景.
대25빈미국감서[Ipomoea batatas (L.) Lam.]품계급령외작위대조적47빈감서충질자원진행ISSR분석,이용NTSYS연건분석유전상사계수,채용UPGMA방법진행취류,구건친연관계계통도.결과현시:10개인물공확증출78개조대,기중유73조정현다태성,다태위점비례고체93.6%;25빈미국감서육충재료여대조충질자원적평균유전상사성계수시0.74.표명미국감서육충재료유전다양성교고,가장기중적6-3-1、6-8-7등품계응용우국내감서육충중,이확대유전배경.
To understand the genetic diversity of sweetpotato (Ipomoea batatas) collected from USA, 72 sweetpota-to breeding lines were analyzed using ISSR markers. The genetic similarity of genetic relationship was calculated by NTSYS software, and dendrogram was clustered by UPGMA method. Based on 73 polymorphic bands amplified from ten primer pairs, genetic similarities among 25 USA lines and 47 control lines ranged from 0. 52 to 0. 94, with an average genetic simi-larity of 0. 74. The results showed that sufficient polymorphism could be found among USA sweetpotato breeding lines, and some lines, such as 6-3-1 and 6-8-7 , could be potential for sweetpotato hybridization breeding.