CAJ | 학술논문

为明确从番茄植株体内筛选出的内生环状芽孢杆菌(Bacillus circulan)Jcxy8对番茄灰霉病菌的抑菌作用及防病的生理生化机制, 采用平板打孔法测定了菌株Jcxy8对灰霉病菌(Botrytis cinerea)的拮抗力.结果表明: 菌株Jcxy8对灰霉病菌的抑菌圈直径为35.6 mm, 抑菌圈边缘的产孢抑制率达到66.9%.当菌株培养滤液浓度为40%时, 病菌孢子萌发完全被抑制.镜检发现抑菌圈周围的菌丝(或芽管)细胞消融, 生长扭曲, 中间或顶端膨大成泡囊状.Jcxy8菌株与灰霉病菌同时处理的番茄果体内可溶性蛋白含量比清水对照处理高12.7%, 比单独接种灰霉病菌处理高39.1%; SOD、POD、CAT活性均较只经病菌处理低;O_2~(1)产生速率比清水对照和病菌处理低, 而比菌株Jcxy8处理高.说明菌株Jcxy8对番茄果实有明显的诱导抗病作用.
위명학종번가식주체내사선출적내생배상아포간균(Bacillus circulan)Jcxy8대번가회매병균적억균작용급방병적생리생화궤제, 채용평판타공법측정료균주Jcxy8대회매병균(Botrytis cinerea)적길항력.결과표명: 균주Jcxy8대회매병균적억균권직경위35.6 mm, 억균권변연적산포억제솔체도66.9%.당균주배양려액농도위40%시, 병균포자맹발완전피억제.경검발현억균권주위적균사(혹아관)세포소융, 생장뉴곡, 중간혹정단팽대성포낭상.Jcxy8균주여회매병균동시처리적번가과체내가용성단백함량비청수대조처리고12.7%, 비단독접충회매병균처리고39.1%; SOD、POD、CAT활성균교지경병균처리저;O_2~(1)산생속솔비청수대조화병균처리저, 이비균주Jcxy8처리고.설명균주Jcxy8대번가과실유명현적유도항병작용.
To explore the inhibiting effect and physiological mechanism of endophytic bacteria (Bacillus circulans) Jcxy8 isolated from tomato against Botrytis cinerea pathogen, a series of analysis were carried out including inhibitory ability analysis via of punching-poles. Results show that Jcxy8 strain inhibits mycelial growth of B. cinerea at an average inhibitory circle diameter of 35.6 mm. Inhibition rate of spore production around inhibitory circles is 66.9%. B. cinerea spores do not germinate when Jcxy8 strain culture filtrate concentration is above 40%. Microscopic observations indicate that mycelial cells around inhibitory circles fade away and twist up with vesicle-like stems or tips. Physiological analysis with spectrophotometer on tomato fruits inoculated with both Jcxy8 and B. cinerea shows increasing soluble protein content, which in is 12.7% higher than that in water, and 39.1% higher than that in fruits inoculated with B. cinerea only and activities of superoxidase (POD), superoxide dismutase (SOD), catalase (CAT) are just lower than inoculated with B. cinerea. It also suppresses the production rate of superoxide anionradicals (O_2~(1)), implying that Jcxy8 strain significantly induces the tomato fruits' resistance on tomato gray mould.