CAJ | 학술논문

为了提高猪成熟卵母细胞细胞玻璃化冷冻效果.本试验拟添加紫杉醇,比较其对冷冻环冷冻效果的影响.结果显示使用1.0μmolL~(-1)紫杉醇预处理卵母细胞,冷冻后其形态完整率(89.93%)和FDA染色存活率(83.33%)都显著高于未处理组的79.12%和70.97%(P＜0.05),继续提高紫杉醇浓度则表现为对卵母细胞的毒副作用.在不同预处理时间上,预处理30 min组冷冻后卵母细胞形态完整率和FDA染色存活率最高,分别达到90.21%和84.13%.预处理浓度1.0μmol·L~(-1),30 min是比较合适的处理方法;紫杉醇、细胞松弛素B(CB)或两者联合添加能显著提高猪成熟卵母细胞的玻璃化冷冻的效果(P＜0.05),但两者之间没有显著差异(P＞0.05).
위료제고저성숙란모세포세포파리화냉동효과.본시험의첨가자삼순,비교기대냉동배냉동효과적영향.결과현시사용1.0μmolL~(-1)자삼순예처리란모세포,냉동후기형태완정솔(89.93%)화FDA염색존활솔(83.33%)도현저고우미처리조적79.12%화70.97%(P＜0.05),계속제고자삼순농도칙표현위대란모세포적독부작용.재불동예처리시간상,예처리30 min조냉동후란모세포형태완정솔화FDA염색존활솔최고,분별체도90.21%화84.13%.예처리농도1.0μmol·L~(-1),30 min시비교합괄적처리방법;자삼순、세포송이소B(CB)혹량자연합첨가능현저제고저성숙란모세포적파리화냉동적효과(P＜0.05),단량자지간몰유현저차이(P＞0.05).
The aim of this study was to improve the effect of vitrification of porcine matured oocyte. This study was designed to examine the effect of Taxol pretreatment on vitrification of porcine oocytes matured in vitro by a cryoloop method. The results showed that the vitrified oocytes pre-treated with 1.0μmol·L~(-1) Taxol attained higher normal morphology rate(89.93%) and FDA stain survival rate(83.33%) than the control(79.12% and 70. 97%) (P＜0.05). But when the concentration of Taxol was increased, it came to weaken the effect of vitrification. The proportions of oocytes with normal morphology and survive rates could reach to 90. 21% and 84.13%,when they were treated for 30 minutes before vitrification. So the best parameter was 1. 0μmol·L~(-1) Taxol for 30 minutes before vitrification. Pre-treatment with CB or Taxol had a significant positive effect on vitrification of porcine matured oocytes(P＜0.05), but there were no significant differences between the two chemical materials(P＞0.05).