CAJ | 학술논문

目的探讨脂联素基因多态性及其单倍型与2型糖尿病(T2DM)的相关性.方法人选T2DM患者202例,糖尿病家族史阴性的非糖尿病个体(NDM)143例.采用聚合酶链反应一限制性内切酶片段长度多态性(PCR-RFLP)方法对脂联素基因SNP-11391、SNP-11377、SNP-4522、SNP+45和SNP+331位点进行基因分型,并构建单倍型.评估以上5个多态性位点及构建的单倍型与T2DM的相关性.结果 T2DM组和NDM组脂联素基因SNP-11391、SNP-11377、SNP-4522、SNP+45和SNP+331基因型和等位基因频率差异无统计学意义.T2DM组和NDM组SNP-11377C-SNP-4522T单倍型频率差异有统计学意义(P<0.05).结论脂联素基因SNP-11377C-SNP-4522T单倍型与T2DM相关,且可能增加患T2DM风险(OR=1.60,95%CI:1.09～2.35).
목적 탐토지련소기인다태성급기단배형여2형당뇨병(T2DM)적상관성.방법 인선T2DM환자202례,당뇨병가족사음성적비당뇨병개체(NDM)143례.채용취합매련반응일한제성내절매편단장도다태성(PCR-RFLP)방법대지련소기인SNP-11391、SNP-11377、SNP-4522、SNP+45화SNP+331위점진행기인분형,병구건단배형.평고이상5개다태성위점급구건적단배형여T2DM적상관성.결과 T2DM조화NDM조지련소기인SNP-11391、SNP-11377、SNP-4522、SNP+45화SNP+331기인형화등위기인빈솔차이무통계학의의.T2DM조화NDM조SNP-11377C-SNP-4522T단배형빈솔차이유통계학의의(P<0.05).결론 지련소기인SNP-11377C-SNP-4522T단배형여T2DM상관,차가능증가환T2DM풍험(OR=1.60,95%CI:1.09～2.35).
Objective　　To investigate the association of single nucleotide polymorphism (SNP) haplotypes in adiponectin gene with type 2 diabetes mellitus (T2DM). Methods The study included 202 patients with T2DM and 143 nondiabetic(NDM) healthy subjects with no family history of diabetes. Using polymerase chain reaction-based restriction fragment-length polymorphism (PCR-RFLP), we genotyped the SNP-11391, SNP-11377, SNP-4522, SNP+45 and SNP+331 in adiponectin gene, and constructed their haplotypes. Then, we assessed the association of the five SNPs with T2DM. Results (1)The genotypic and allelic frequencies of SNP-11391, SNP-11377, SNP-4522, SNP+45 and SNP+331 had no significant difference between T2DM and NDM groups (all P>0.05). (2)The frequencies of haplotype SNP-11377C-SNP-4522T had difference between T2DM and NDM groups(P=0.02). Conclusions The haplotype SNP-11377C-SNP-4522T of adiponectin gene is associated with increased T2DM risk (Or=1.60, 95%CI:1.09~2.35).　　