CAJ | 학술논문

背景:以往支架材料修复骨软骨的实验大都存在骨软骨耦合界面修复不良的情况.目的:观察骨髓间充质干细胞/软骨细胞复合动物源性骨软骨支架修复兔膝关节骨软骨复合缺损的可行性.方法:将新西兰大白兔随机抽签分为实验组、对照组、空白组,制作单侧膝关节骨软骨复合缺损后,实验组于骨缺损处植入自体骨髓间充质干细胞/诱导分化的软骨细胞与同种异体动物源性骨软骨复合支架,对照组于骨缺损处植入同种异体动物源性骨软骨支架、空白组未植入任何材料.术后4,8,12周行大体观察、苏木精-伊红染色、甲苯胺蓝染色.结果与结论:实验组大体观察见复合缺损区完全修复,局部无凹陷,新生组织和周围组织融合,苏木精-伊红染色和甲苯胺蓝染色见软骨缺损区由新生的透明软骨样组织修复,细胞柱状排列,极性好,软骨陷窝明显,骨缺损区由骨样组织修复,新生软骨和软骨下骨以及宿主骨界面耦合良好,甲苯胺蓝染色阳性率和组织学评分优于对照组、空白组(P < 0.05).说明诱导分化的自体软骨细胞和骨髓间充质干细胞共培养复合动物源性骨软骨支架所构建的细胞-支架复合体能成功修复兔膝关节软骨和软骨下骨的复合缺损,是一种理想的骨软骨复合缺损修复方法.
배경:이왕지가재료수복골연골적실험대도존재골연골우합계면수복불량적정황.목적:관찰골수간충질간세포/연골세포복합동물원성골연골지가수복토슬관절골연골복합결손적가행성.방법:장신서란대백토수궤추첨분위실험조、대조조、공백조,제작단측슬관절골연골복합결손후,실험조우골결손처식입자체골수간충질간세포/유도분화적연골세포여동충이체동물원성골연골복합지가,대조조우골결손처식입동충이체동물원성골연골지가、공백조미식입임하재료.술후4,8,12주행대체관찰、소목정-이홍염색、갑분알람염색.결과여결론:실험조대체관찰견복합결손구완전수복,국부무요함,신생조직화주위조직융합,소목정-이홍염색화갑분알람염색견연골결손구유신생적투명연골양조직수복,세포주상배렬,겁성호,연골함와명현,골결손구유골양조직수복,신생연골화연골하골이급숙주골계면우합량호,갑분알람염색양성솔화조직학평분우우대조조、공백조(P < 0.05).설명유도분화적자체연골세포화골수간충질간세포공배양복합동물원성골연골지가소구건적세포-지가복합체능성공수복토슬관절연골화연골하골적복합결손,시일충이상적골연골복합결손수복방법.
BACKGROUND: Though there were many experiments addressing repairing osteochondral defects before, faulty restoration occurred at coupling interfaces. OBJECTIVE: To investigate the feasibility of repairing of osteochondral composite defects in rabbit knees with animal-origin osteochondral scaffold combined with bone marrow mesenchymal stem cells (BMSCs)/chondrocytes.METHODS: New Zealand white rabbits were randomly divided into the experimental, control and blank groups and prepared for unilateral knee joint osteochondral defects. Animal-origin osteochondral scaffold combined with BMSCs/chondrocytes, animal-origin osteochondral scaffold and no material was implanted to repair the defects in the experimental, control and blank groups, respectively. Healing condition was evaluated by gross observation, hematoxylin-eosin staining, and toluidine blue staining at 4, 8, and 12 weeks after operation. RESULTS AND CONCLUSION: At 12 weeks after operation, gross observation showed the defects were repaired completely without local depression and the regenerated tissues were fused with surrounding tissues in the experimental group. Hematoxylin-eosin staining and toluidine blue staining revealed that there were many new hyaline cartilages in the cartilage defects in which columnar cells were lined well and cartilage lacuna was obviously, also, there were many bony tissues in the bone defects. The regeneration cartilage, the underlying subchondral bone and host bone were coupled completely. The toluidine blue positive rate and histologic scores of the experimental group were superior to those of the control and blank groups (P < 0.05). It is demonstrated that animal-origin osteochondral scaffold combined with BMSCs/chondrocytes is an ideal method to repair defects between cartilage and the underlying subchondral bone.