CAJ | 학술논문

目的:通过分析健康受试者口服六味地黄丸后数个重要穴位激光多普勒血流信号的频谱成分,了解口服六味地黄丸对体表微循环血流的影响.方法:对两组男性健康受试者进行激光多普勒血流信号测量.六味地黄丸实验组42名受试者,平均年龄(27.8±3.8)岁;空白实验组28名受试者,平均年龄(27.3±3.4)岁.每次测量实验包含一次20 min的基础测量与3次药效测量,其中两次于服药当天进行(口服5 g六味地黄丸的浓缩药粉,配合250 mL 28℃温水服用),另一次测量于服药后第2天进行.结果:在服药后第2天,太溪穴处测量到最明显的平均血流量与脉波血流量增大,此外由频谱分析可发现,0.02～0.06 Hz频带的能量比例显著降低,而在其他测量位置,则无任何显著的效应.结论:在口服六味地黄丸之后,体表微循环血流会进行重新分配调整,这可能因于不同体表穴位的血管床和交感神经作用强度不同从而引发微动脉小开口的血流阻力不同.这一非侵入式与实时监控的技术,有助于证实中药的生理效应及其生理作用机制.
목적:통과분석건강수시자구복륙미지황환후수개중요혈위격광다보륵혈류신호적빈보성분,료해구복륙미지황환대체표미순배혈류적영향.방법:대량조남성건강수시자진행격광다보륵혈류신호측량.륙미지황환실험조42명수시자,평균년령(27.8±3.8)세;공백실험조28명수시자,평균년령(27.3±3.4)세.매차측량실험포함일차20 min적기출측량여3차약효측량,기중량차우복약당천진행(구복5 g륙미지황환적농축약분,배합250 mL 28℃온수복용),령일차측량우복약후제2천진행.결과:재복약후제2천,태계혈처측량도최명현적평균혈류량여맥파혈류량증대,차외유빈보분석가발현,0.02～0.06 Hz빈대적능량비례현저강저,이재기타측량위치,칙무임하현저적효응.결론:재구복륙미지황환지후,체표미순배혈류회진행중신분배조정,저가능인우불동체표혈위적혈관상화교감신경작용강도불동종이인발미동맥소개구적혈류조력불동.저일비침입식여실시감공적기술,유조우증실중약적생리효응급기생리작용궤제.
This study aimed to assess the skin-surface microcirculatory effects of oral administration of Liuwei Dihuang (LW) formula by analyzing the frequency content of laser-Doppler skin bloodflow signals at important acupoints.Methods:Laser-Doppler signals were measured in male healthy volunteers in two groups of experiments:the LW experiment (n =42; aged (27.8± 3.8) years) and the control experiment (n=28; aged (27.3±3.4) years).Each experiment involved a 20-minute baseline-data sequence and three following sets of effects data:recorded on the same day after oral administration of 250 mL water (28 ℃ ) accompanied with 5 g of LW formula (herbal extract granules),and recorded on the second day after taking other two doses of LW formula.Results:During the second day,the increases in the mean and pulsatile components of the flux signal were the most prominent,and the relative energy contribution at 0.02 to 0.06 Hz was significantly decreased at Taixi (K13).There were no any other significant changes in these parameters at other measurement sites.Conclusion:The redistribution of skin-surface microcirculatory blood flow following oral LW administration observed here can be attributed to different changes in sympathetic neural activities and thus the different perfusion resistance through the arteriolar openings of the peripheral vascular beds at different measurement sites.The developed noninvasive and real-time monitoring technique could form part of an evidence-based method for studying the physiological effects and the underlying mechanisms of traditional Chinese medicine herbs.