国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2011年
11期
834-838
,共5页
慢性阻塞性肺疾病%白介素17%肿瘤坏死因子α%气道重塑
慢性阻塞性肺疾病%白介素17%腫瘤壞死因子α%氣道重塑
만성조새성폐질병%백개소17%종류배사인자α%기도중소
Chronic obstructive pulmonary disease%Interleukin-17%Tumor necrosis factor alpha%Airway remodeling
目的 探讨白介素17(IL-17)在慢性阻塞性肺疾病(COPD)大鼠气道炎症及重塑中的作用.方法 采用被动吸烟80 d来制备大鼠COPD模型.支气管肺泡灌洗计数支气管肺泡灌洗液(BALF)中细胞计数和百分比;采用半定量图像分析法测量小气道管壁和平滑肌层厚度;免疫组织化学法检测肺组织IL-17的表达;酶联免疫吸附试验法检测肺组织匀浆中IL-17及血清、BALF中肿瘤坏死因子α(TNF-α)的含量.结果 采用熏烟法成功复制了大鼠COPD模型.COPD组IL-17在支气管上皮细胞内呈强阳性表达,肺组织匀浆IL-17含量显著高于对照组(P<0.01),与BALF中中性粒细胞数及TNF-α的含量呈正相关(r=0.65,P<0.05;r=0.76,P<0.01),与小气道管壁和平滑肌层厚度呈正相关(r=0.89,P<0.01;r=0.80,P<0.01).结论 COPD中IL-17可能通过增强TNF-α的表达等途径促进中性粒细胞在气道内聚集,引起支气管管壁纤维性增生增厚及平滑肌层增厚,参与对COPD气道炎症和气道重塑调节.
目的 探討白介素17(IL-17)在慢性阻塞性肺疾病(COPD)大鼠氣道炎癥及重塑中的作用.方法 採用被動吸煙80 d來製備大鼠COPD模型.支氣管肺泡灌洗計數支氣管肺泡灌洗液(BALF)中細胞計數和百分比;採用半定量圖像分析法測量小氣道管壁和平滑肌層厚度;免疫組織化學法檢測肺組織IL-17的錶達;酶聯免疫吸附試驗法檢測肺組織勻漿中IL-17及血清、BALF中腫瘤壞死因子α(TNF-α)的含量.結果 採用熏煙法成功複製瞭大鼠COPD模型.COPD組IL-17在支氣管上皮細胞內呈彊暘性錶達,肺組織勻漿IL-17含量顯著高于對照組(P<0.01),與BALF中中性粒細胞數及TNF-α的含量呈正相關(r=0.65,P<0.05;r=0.76,P<0.01),與小氣道管壁和平滑肌層厚度呈正相關(r=0.89,P<0.01;r=0.80,P<0.01).結論 COPD中IL-17可能通過增彊TNF-α的錶達等途徑促進中性粒細胞在氣道內聚集,引起支氣管管壁纖維性增生增厚及平滑肌層增厚,參與對COPD氣道炎癥和氣道重塑調節.
목적 탐토백개소17(IL-17)재만성조새성폐질병(COPD)대서기도염증급중소중적작용.방법 채용피동흡연80 d래제비대서COPD모형.지기관폐포관세계수지기관폐포관세액(BALF)중세포계수화백분비;채용반정량도상분석법측량소기도관벽화평활기층후도;면역조직화학법검측폐조직IL-17적표체;매련면역흡부시험법검측폐조직균장중IL-17급혈청、BALF중종류배사인자α(TNF-α)적함량.결과 채용훈연법성공복제료대서COPD모형.COPD조IL-17재지기관상피세포내정강양성표체,폐조직균장IL-17함량현저고우대조조(P<0.01),여BALF중중성립세포수급TNF-α적함량정정상관(r=0.65,P<0.05;r=0.76,P<0.01),여소기도관벽화평활기층후도정정상관(r=0.89,P<0.01;r=0.80,P<0.01).결론 COPD중IL-17가능통과증강TNF-α적표체등도경촉진중성립세포재기도내취집,인기지기관관벽섬유성증생증후급평활기층증후,삼여대COPD기도염증화기도중소조절.
Objective To study the role of interleukin-17 (IL-17) in the airway inflammation and remodeling of rats with chronic obstructive pulmonary disease (COPD). Methods Rat models of COPD were replicated by passive smoking for 80 days. Total cell counts and neutrophil counts in bronchoalveolar lavage fluid (BALF) were examined. The thickness of the airway wall and the smooth muscle layer of the small airway were measured by means of image analyzer. The expression of IL-17 in rat lung tissue was detected by immunohistochemistry. The concentrations of IL-17 in lung tissues and tumor necrosis factorα(TNF-α) in BALF and serum were measured with enzyme-linked immunosorbent assay. Results Rat models of COPD were replicated successfully by passive smoking. IL-17 was mainly expressed in airway epithelial cells of COPD rats. The concentrations of IL-17 in lung tissue were significantly higher in the COPD group than in the normal group( P <0. 01). In the COPD group,IL-17 concentration of lung tissue was positively correlated with the neutrophil counts and TNF-α level in BALF( r =0. 65, P <0. 05. r =0. 76, P <0. 01), it also tended to be positively correlated with the thickness of the airway wall and the smooth muscle layer of the small airwayC r =0. 89, P <0. 01. r =0. 80, P <0. 01 ). Conclusions IL-17 probably recruits neutrophils into airways by enhancing the expression of TNF-α,and it may play a role in the airway inflammation and remodeling of COPD.
