中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2010年
12期
1096-1098
,共3页
金曼%吕佩源%李玲%董艳红%岳洪娟%李金玲%范鸣玥
金曼%呂珮源%李玲%董豔紅%嶽洪娟%李金玲%範鳴玥
금만%려패원%리령%동염홍%악홍연%리금령%범명모
阿尔茨海默病%Aβ1-42%一氧化碳%SN56细胞%存活率
阿爾茨海默病%Aβ1-42%一氧化碳%SN56細胞%存活率
아이자해묵병%Aβ1-42%일양화탄%SN56세포%존활솔
Alzheimer's disease%Aβ1-42%Carbon monoxide%SN56 cells%Livability
目的 观察低浓度Aβ1-42单体/寡聚体对胆碱能SN56细胞存活率的影响以及不同浓度CORM-2的作用.方法 将相同细胞浓度的SN56细胞培养在96-孔细胞培养板中并将细胞分为对照组、Aβ1-42组、Aβ1-42+CORM-2 50μM组及Aβ1-42+CORM-2 100μM组.Aβ1-42组三列细胞分别在10nM、100nM及1μM Aβ1-42单体/寡聚体的环境中培养.Aβ1-42+CORM-2 50μM组和Aβ1-42+CORM-2 100μM组细胞除每孔L分别含50μM及100μM CORM-2外,其余培养条件与Aβ1-42组相同.对照组不加任何处理因素.培养3d后,用MTT法比较不同组别细胞存活率.结果 Aβ1-42组细胞存活率分别为(79.73±0.94)%、(67.99±0.79)%、(60.42 ±0.62)%,随Aβ1-42浓度增加而下降.Aβ1-42+CORM-2 50μM/100μM组细胞存活率分别为(75.15±0.096)%、(63.20±0.17)%、(55.33±0.19)%;(73.20±0.27)%、(64.34±0.11)%、(54.17 ±0.12)%,均低于单纯Aβ1-42组;并且不同CORM-2浓度之间,降低细胞存活率的作用存在差异.结论 低浓度Aβ1-42单体/寡聚体可降低SN56细胞的存活率,并且浓度越高,效果越明显;不同浓度CORM-2均可使SN56细胞的存活率下降,且作用强度存在差异.
目的 觀察低濃度Aβ1-42單體/寡聚體對膽堿能SN56細胞存活率的影響以及不同濃度CORM-2的作用.方法 將相同細胞濃度的SN56細胞培養在96-孔細胞培養闆中併將細胞分為對照組、Aβ1-42組、Aβ1-42+CORM-2 50μM組及Aβ1-42+CORM-2 100μM組.Aβ1-42組三列細胞分彆在10nM、100nM及1μM Aβ1-42單體/寡聚體的環境中培養.Aβ1-42+CORM-2 50μM組和Aβ1-42+CORM-2 100μM組細胞除每孔L分彆含50μM及100μM CORM-2外,其餘培養條件與Aβ1-42組相同.對照組不加任何處理因素.培養3d後,用MTT法比較不同組彆細胞存活率.結果 Aβ1-42組細胞存活率分彆為(79.73±0.94)%、(67.99±0.79)%、(60.42 ±0.62)%,隨Aβ1-42濃度增加而下降.Aβ1-42+CORM-2 50μM/100μM組細胞存活率分彆為(75.15±0.096)%、(63.20±0.17)%、(55.33±0.19)%;(73.20±0.27)%、(64.34±0.11)%、(54.17 ±0.12)%,均低于單純Aβ1-42組;併且不同CORM-2濃度之間,降低細胞存活率的作用存在差異.結論 低濃度Aβ1-42單體/寡聚體可降低SN56細胞的存活率,併且濃度越高,效果越明顯;不同濃度CORM-2均可使SN56細胞的存活率下降,且作用彊度存在差異.
목적 관찰저농도Aβ1-42단체/과취체대담감능SN56세포존활솔적영향이급불동농도CORM-2적작용.방법 장상동세포농도적SN56세포배양재96-공세포배양판중병장세포분위대조조、Aβ1-42조、Aβ1-42+CORM-2 50μM조급Aβ1-42+CORM-2 100μM조.Aβ1-42조삼렬세포분별재10nM、100nM급1μM Aβ1-42단체/과취체적배경중배양.Aβ1-42+CORM-2 50μM조화Aβ1-42+CORM-2 100μM조세포제매공L분별함50μM급100μM CORM-2외,기여배양조건여Aβ1-42조상동.대조조불가임하처리인소.배양3d후,용MTT법비교불동조별세포존활솔.결과 Aβ1-42조세포존활솔분별위(79.73±0.94)%、(67.99±0.79)%、(60.42 ±0.62)%,수Aβ1-42농도증가이하강.Aβ1-42+CORM-2 50μM/100μM조세포존활솔분별위(75.15±0.096)%、(63.20±0.17)%、(55.33±0.19)%;(73.20±0.27)%、(64.34±0.11)%、(54.17 ±0.12)%,균저우단순Aβ1-42조;병차불동CORM-2농도지간,강저세포존활솔적작용존재차이.결론 저농도Aβ1-42단체/과취체가강저SN56세포적존활솔,병차농도월고,효과월명현;불동농도CORM-2균가사SN56세포적존활솔하강,차작용강도존재차이.
Objective To observe the effect of low concentration Aβ1-42 monomer/oligomers and CORM-2 in different concentration on livability of SN56 cells. Methods SN56 cells were cultured in the 96-well plate with uniform concentration, and were divided into control group, Aβ1-42 group, Aβ1-42 + CORM-2 50μM group, and Aβ1-42 + CORM-2 100 μM group. Three lines of cells in Aβ1-42 group were cultured in the surroundings of 10nM,100nM and 1 μM Aβ1-42monomer/oligomers, respectively. Aβ1-42 + CORM-2 50μM group and Aβ1-42 + CORM-2 100μM group had the same culture condition as group Aβ1-42 ,except contain 50μM, and 100μM CORM-2, respectively. Control group didnt have any effect factor. Three days later,the livability of different groups was compared with MTT method. Results The livability of group Aβ1-42 with the increasing concentration of Aβ1-42 was (79.73 ±0.94)% ,(67.99 ±0.79)% ,(60.42 ±0.62)% , respectively. The higher the concentration of Aβ1-42 was,the lower the livability of SN56 cell was. The livability of group Aβ1-42 + CORM-2 50μM/100μM was( 75.15±0.096)%,(63.20 ±0.17)%, (55.33 ±0.19)%; (73.20 ±0.27)%, (64.34 ±0.11 )%, (54.17 ±0.12)% , respectively. Both were lower than group Aβ1-42. And different CORM-2 concentration had discrepancy in the ability of decreasing the cell livability. Conclusion Low concentration of Aβ1-42 can reduce the livability of SN56 cells, and higher concentration has more significant effect; CORM-2 in different concentration both can decrease the livability of SN56 cells,and there is a discrepancy in the intensity.
