CAJ | 학술논문

目的探讨短期过量饮酒对大鼠心功能的影响机制以及血管紧张素Ⅱ受体拮抗剂(缬沙坦)的保护作用.方法 20周龄、雄性Wistar大鼠42只随机分成4组,对照组10只、酒精组10只、低剂量缬沙坦组(LD组)11只和高剂量缬沙坦组(HD组)11只;各组喂养饲料相同,对照组给予清水灌胃,其余3组给予浓度40%(V/V)食用洒灌胃(6.4 g/kg),LD(15 mg/kg)和HD组(30 mg/kg)再给予不同剂量的缬沙坦.9周后,应用超声心动图观察大鼠心功能的变化,称量体质量(BW)和全心质量(HW),应用样本碱水解法测定大鼠心肌羟脯氨酸含量.结果 9周后超声心动显示,酒精组心脏收缩功能左室射血分数(LVEF)、短轴缩短率(FS)、每搏输出量(SV)与对照组、LD组、HD组比较,差异无统计学意义(P＞0.05);左室舒张早期血流充盈速度(E)及多普勒超声显像二尖瓣内环舒张早期运动速度(Ea)、舒张晚期运动速度(Aa)和舒张早期晚期速度比(Ea/Aa)明显下降,与对照组、LD组、HD组比较差异有统计学意义(P＜0.05),对照组E峰、Ea/Aa高于LD组和HD组(P＜0.05);酒精组的HW/BW、心肌羟脯氨酸含量明显高于其他各组(P＜0.01),而对照组、LD组和HD组间比较,差异无统计学意义(P＞0.05).结论短期过量饮酒可引起大鼠心肌损伤,导致心脏舒张功能降低,血管紧张索Ⅱ受体拮抗剂缬沙坦对其具有保护作用.
목적 탐토단기과량음주대대서심공능적영향궤제이급혈관긴장소Ⅱ수체길항제(힐사탄)적보호작용.방법 20주령、웅성Wistar대서42지수궤분성4조,대조조10지、주정조10지、저제량힐사탄조(LD조)11지화고제량힐사탄조(HD조)11지;각조위양사료상동,대조조급여청수관위,기여3조급여농도40%(V/V)식용쇄관위(6.4 g/kg),LD(15 mg/kg)화HD조(30 mg/kg)재급여불동제량적힐사탄.9주후,응용초성심동도관찰대서심공능적변화,칭량체질량(BW)화전심질량(HW),응용양본감수해법측정대서심기간포안산함량.결과 9주후초성심동현시,주정조심장수축공능좌실사혈분수(LVEF)、단축축단솔(FS)、매박수출량(SV)여대조조、LD조、HD조비교,차이무통계학의의(P＞0.05);좌실서장조기혈류충영속도(E)급다보륵초성현상이첨판내배서장조기운동속도(Ea)、서장만기운동속도(Aa)화서장조기만기속도비(Ea/Aa)명현하강,여대조조、LD조、HD조비교차이유통계학의의(P＜0.05),대조조E봉、Ea/Aa고우LD조화HD조(P＜0.05);주정조적HW/BW、심기간포안산함량명현고우기타각조(P＜0.01),이대조조、LD조화HD조간비교,차이무통계학의의(P＞0.05).결론 단기과량음주가인기대서심기손상,도치심장서장공능강저,혈관긴장색Ⅱ수체길항제힐사탄대기구유보호작용.
Objective To investigate the mechanism of the effects of heavy alcohol consumption in a short term and the protection of angiotensin Ⅱ receptor blocker (valsartan) on cardiac function in rats. Methods The 42 male Wistar rats aged 20 weeks were randomly divided into 4 groups:control group (group C, n= 10), alcohol group (group A, n=10), low-dose valsartan group (LD group, n= 11) and high-dose valsartan group (HD group, n= 11). They were supplied with same animal feeds, but all of them were administered different dose of alcohol and medicine via intragastric tube: group C was administered water, group A was administered alcohol (6. 4 g/kg), LD group was administered alcohol (6.4 g/kg) and valsartan (15 mg/kg), HD group was administered alcohol (6.4 g/kg) and valsartan (30 mg/kg). And 9 weeks later, the change of cardiac function was observed by echocardiography, the body and heart weight were measured, the hydroxyproline content of rat myocardium was determined by sample alkaline solution. Results After 9 weeks, there were no significant differences among four groups in left ventricular ejection fraction (LVEF), stroke volume (SV) and fraction shortening (FS). But the E peak, Ea/Aa, Ea peak and Aa peak were obviously lower in group A than in groups of C, LD and HD (all P＜0.05), and there were significant differences among C group, LD group and HD group in E peak, Ea/Aa (all P＜0. 05). The HW/BW and hydroxproline (Hyp) contents of myocardium were higher in group A than in groups of C, LD and HD (all P＜0. 01), but there were no statistical significances among group C, LD group and HD group (all P＞0. 05). Conclusions The short term heavy alcohol consumption results in impaired ultrastructure and diastolic function of myocardium in rats, the angiotensin Ⅱ receptor blocker (valsartan) may protect it.