CAJ | 학술논문

目的观察新型多聚体微泡携带舒尼替尼对人肾癌GRC-1细胞生长及凋亡的影响.方法将体外培养的人肾癌GRC-1细胞随机分为6组:空白对照组、单纯微泡组、单纯脂质体组、舒尼替尼组、新型多聚体微泡载舒尼替尼不联合超声组、新型多聚体微泡载舒尼替尼联合超声组.MTT法观察不同处理组细胞生存率,Sigma-FITC荧光染色及透射电镜检测细胞凋亡.结果新型多聚体微泡载舒尼替尼联合超声组对人肾癌GRC-1细胞的生长抑制及促进凋亡作用强于其他处理组及对照组.结论新型多聚体微泡携带舒尼替尼在超声作用下对人肾癌GRC-1细胞生长有明显抑制作用,并诱导细胞凋亡.
목적 관찰신형다취체미포휴대서니체니대인신암GRC-1세포생장급조망적영향.방법 장체외배양적인신암GRC-1세포수궤분위6조:공백대조조、단순미포조、단순지질체조、서니체니조、신형다취체미포재서니체니불연합초성조、신형다취체미포재서니체니연합초성조.MTT법관찰불동처리조세포생존솔,Sigma-FITC형광염색급투사전경검측세포조망.결과 신형다취체미포재서니체니연합초성조대인신암GRC-1세포적생장억제급촉진조망작용강우기타처리조급대조조.결론 신형다취체미포휴대서니체니재초성작용하대인신암GRC-1세포생장유명현억제작용,병유도세포조망.
Objective To investigate the effect of growth inhibition and apoptosis induction of sunitinib-liposome-loaded microbubbles on renal carcinoma cell strain.Methods GRC-1 cell strain was cultured in vitro,and was divided into 6 groups:blank control group,pure microbubbles group,pure lipsomes group,sunitinib group,sunitinib-liposome-loaded microbubbles without ultrasound treat group,sunitinib liposome-loaded microbubbles with ultrasound treat group.Growth inhibition in different groups was observed at different time with MTT assay,apoptosis induction with Sigma-FlTC technology and transmission electron microscope.Results The growth inhibition and apoptosis promotion of GRC-1 cell were significantly increased in sunitinib-liposome-loaded microbubbles with ultrasound treat group compared to the other groups.Conclusions Microbubble guided sunitinih delivery can increase the effect of the growth inhibition and apoptosis induction of GRC-1 cells,which may provide an more effective approach for cancer treatment.