CAJ | 학술논문

目的探讨载bFGF基因腺相关病毒(rAAV2-bFGF)感染后的骨髓间充质干细胞与PDLLLA/HA的复合情况,为颅颌面骨基因治疗提供实验依据.方法 rAAV2-bFGF转染至第3代骨髓间充质干细胞,在无菌情况下将bFGF基因腺相关病毒(rAAV2-bFGF)感染后的骨髓间充质干细胞与聚D,L-乳酸/羟基磷灰石(PDLLA/HA)支架材料充分混匀,4℃过夜,在扫描电镜下观察载骨髓间充质干细胞与支架材料的复合情况.结果 rAAV2-bFGF成功转染至第3代骨髓间充质干细胞；扫描电镜下观察发现,BMSCs可以在PDLLA/HA上黏附、增殖、分泌细胞外基质.结论载bFGF基因腺相关病毒(rAA V2-bFGF)感染后的骨髓间充质于细胞可以在合成的PDLLA/HA上黏附、增殖、分泌细胞外基质,PDLLA/HA可以作为转基因细胞的支架材料.
목적 탐토재bFGF기인선상관병독(rAAV2-bFGF)감염후적골수간충질간세포여PDLLLA/HA적복합정황,위로합면골기인치료제공실험의거.방법 rAAV2-bFGF전염지제3대골수간충질간세포,재무균정황하장bFGF기인선상관병독(rAAV2-bFGF)감염후적골수간충질간세포여취D,L-유산/간기린회석(PDLLA/HA)지가재료충분혼균,4℃과야,재소묘전경하관찰재골수간충질간세포여지가재료적복합정황.결과 rAAV2-bFGF성공전염지제3대골수간충질간세포；소묘전경하관찰발현,BMSCs가이재PDLLA/HA상점부、증식、분비세포외기질.결론 재bFGF기인선상관병독(rAA V2-bFGF)감염후적골수간충질우세포가이재합성적PDLLA/HA상점부、증식、분비세포외기질,PDLLA/HA가이작위전기인세포적지가재료.
Objective To provide experimental support for the start of gene therapy for craniofacial bones by exploring the composition of PDLLA/HA with BMSCs after transfection with rAAV2-bFGF.Methods rAAV2-bFGF was transfected to the third generation of BMSCs.The cells were then fully mixed with PDLLA/HA and cultured overnight at 4℃.The composition of BMSCs with PDLLA/HA was observed under a scanning electron microscope.Results rAAV2-bFGF was successfully transfected to the third generation of BMSCs.The BMSCs could attach and proliferate on PDLLA/HA and secrete extracellular matrix.Conclusions BMSCs carrying rAAV2-mediated bFGF gene can attach and proliferate on PDLLA/HA and secrete extracellular matrix.PDLLA/HA can be used as ascaffold material of transgenic cells.