CAJ | 학술논문

目的研究正己烷染毒对大鼠视网膜组织的损伤.方法将32只雄性SD大鼠随机分成对照组和正己烷染毒组.对照组:8只,吸人空气;染毒组:24只,采取静式吸入染毒方式,正己烷浓度为35.2 g/m3,每组6只,分别染毒1、3、7、14 d后处死大鼠.采用光学显微镜和透射电子显微镜观察正已烷对视网膜组织病理学和超微结构的影响.结果对照组大鼠视网膜组织结构层次清楚,染色均匀,细胞形态规整.染毒组大鼠正己烷染毒7 d和14d时视网膜出现明显变性,并随染毒时间延长损伤加重.染毒14d,大鼠光感受器外节失去正常的膜盘结构,部分膜盘溶解;内节线粒体肿胀甚至消失.外核层细胞水肿,核内染色质浓缩.内核层水平细胞、双极细胞和无长突细胞出现明显变性.节细胞胞质水肿,细胞器明显减少.外丛状层、内丛状层和神经纤维层神经突起水肿,突触内微丝和小泡减少.结论正己烷可造成大鼠视网膜组织的明显损害,将导致眼底病的发生.
목적 연구정기완염독대대서시망막조직적손상.방법 장32지웅성SD대서수궤분성대조조화정기완염독조.대조조:8지,흡인공기;염독조:24지,채취정식흡입염독방식,정기완농도위35.2 g/m3,매조6지,분별염독1、3、7、14 d후처사대서.채용광학현미경화투사전자현미경관찰정이완대시망막조직병이학화초미결구적영향.결과 대조조대서시망막조직결구층차청초,염색균균,세포형태규정.염독조대서정기완염독7 d화14d시시망막출현명현변성,병수염독시간연장손상가중.염독14d,대서광감수기외절실거정상적막반결구,부분막반용해;내절선립체종창심지소실.외핵층세포수종,핵내염색질농축.내핵층수평세포、쌍겁세포화무장돌세포출현명현변성.절세포포질수종,세포기명현감소.외총상층、내총상층화신경섬유층신경돌기수종,돌촉내미사화소포감소.결론 정기완가조성대서시망막조직적명현손해,장도치안저병적발생.
Objective To investigate the injury in the retina of rats exposed to n-hexane. Methods Thirty-two SD male rats were randomly divided into control group and four n-hexane groups. The rats in the four n-hexane groups inhaled 35.2 g/m3 n-hexane statically for 1, 3, 7 and 14 days respectively (6 rats in ev-ery group)while 8 rats in the control group inhaled air. Histopathology and ultrastrueture changes of the retina of rats were analyzed. Results Rats in control group had clear layers of retinal structure, stained evenly and with regular cell shape. Retinal degeneration was observed in the rats exposed to n-hexane for 7 d and 14 d, and aggravated by degrees with time exposed to n-hexane. In the rats exposed to n-hexane for 14 d, the outer segments of photoreceptor were arranged in a confusing order, and topically there appeared dissolution; in the inner segments, mitochondria were swollen or disappeared. Pyknotie ehromatin and cytoplasmic edema were observed in the outer nuclear layer. There were degeneration of horizontal cells, bipolar cells and amacrine cells in the inner nuclear layer. Cytoplasmic edema and organelle dissolution were observed in ganglionic cells. In the neurofibrous layer, outer and inner plexiform layers, there was neuron cell tuber edema, and the micro-filament and racuole of synapse decreased. Conclusion The histopathology and ultrastructure of retina are damaged in the rats exposed to n-hexane, thus leading to ocular fundus disease.