中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2012年
10期
738-741
,共4页
王宁%鲁艳明%王诗卓%张乔%肖倩%李威%魏恒%银铎%欧阳玲%张淑兰
王寧%魯豔明%王詩卓%張喬%肖倩%李威%魏恆%銀鐸%歐暘玲%張淑蘭
왕저%로염명%왕시탁%장교%초천%리위%위항%은탁%구양령%장숙란
乳头状瘤病毒感染%子宫颈%干扰素Ⅱ型%多态性,单核苷酸%疾病遗传易感性
乳頭狀瘤病毒感染%子宮頸%榦擾素Ⅱ型%多態性,單覈苷痠%疾病遺傳易感性
유두상류병독감염%자궁경%간우소Ⅱ형%다태성,단핵감산%질병유전역감성
Papillomavirus infections%Cervix uteri%Interferon type Ⅱ%Polymorphism,single nucleotide%Genetic predisposition to disease
目的 探讨γ干扰素基因多态性与宫颈HPV感染及HPV感染预后的相关性.方法 应用等位基因特异性引物PCR(PCR-ASP)技术对宫颈HPV阳性患者(观察组,n=179)和宫颈HPV阴性女性(对照组,n=328)γ 干扰素基因rs2430561位点的基因型进行检测.结果 观察组A等位基因频率为63.7％(228/358),明显高于T等位基因频率36.3％(130/358),差异有统计学意义(P=0.045).观察组AA基因型频率为41.3％(74/179),TT基因型频率为14.0％ (25/179),AA基因型女性宫颈HPV感染风险明显高于TT基因型者(OR=1.784,95％ CI为1.031 ～3.088,P=0.039).随访期间,AA基因型宫颈HPV再次检出率为83.8％ (62/74),而TT基因型为20.0％(5/25).Kaplan-Meier分析结果显示,AA、TA、TT基因型HPV感染累积阴性率有明显差异(P =0.008),AA基因型女性HPV感染的累积阴性率最低(1.1％～5.9％).结论 γ干扰素基因rs2430561位点多态性影响女性宫颈HPV的易感性,AA基因型女性感染风险更高,并易于HPV感染的持续和复发.
目的 探討γ榦擾素基因多態性與宮頸HPV感染及HPV感染預後的相關性.方法 應用等位基因特異性引物PCR(PCR-ASP)技術對宮頸HPV暘性患者(觀察組,n=179)和宮頸HPV陰性女性(對照組,n=328)γ 榦擾素基因rs2430561位點的基因型進行檢測.結果 觀察組A等位基因頻率為63.7％(228/358),明顯高于T等位基因頻率36.3％(130/358),差異有統計學意義(P=0.045).觀察組AA基因型頻率為41.3％(74/179),TT基因型頻率為14.0％ (25/179),AA基因型女性宮頸HPV感染風險明顯高于TT基因型者(OR=1.784,95％ CI為1.031 ～3.088,P=0.039).隨訪期間,AA基因型宮頸HPV再次檢齣率為83.8％ (62/74),而TT基因型為20.0％(5/25).Kaplan-Meier分析結果顯示,AA、TA、TT基因型HPV感染纍積陰性率有明顯差異(P =0.008),AA基因型女性HPV感染的纍積陰性率最低(1.1％～5.9％).結論 γ榦擾素基因rs2430561位點多態性影響女性宮頸HPV的易感性,AA基因型女性感染風險更高,併易于HPV感染的持續和複髮.
목적 탐토γ간우소기인다태성여궁경HPV감염급HPV감염예후적상관성.방법 응용등위기인특이성인물PCR(PCR-ASP)기술대궁경HPV양성환자(관찰조,n=179)화궁경HPV음성녀성(대조조,n=328)γ 간우소기인rs2430561위점적기인형진행검측.결과 관찰조A등위기인빈솔위63.7％(228/358),명현고우T등위기인빈솔36.3％(130/358),차이유통계학의의(P=0.045).관찰조AA기인형빈솔위41.3％(74/179),TT기인형빈솔위14.0％ (25/179),AA기인형녀성궁경HPV감염풍험명현고우TT기인형자(OR=1.784,95％ CI위1.031 ～3.088,P=0.039).수방기간,AA기인형궁경HPV재차검출솔위83.8％ (62/74),이TT기인형위20.0％(5/25).Kaplan-Meier분석결과현시,AA、TA、TT기인형HPV감염루적음성솔유명현차이(P =0.008),AA기인형녀성HPV감염적루적음성솔최저(1.1％～5.9％).결론 γ간우소기인rs2430561위점다태성영향녀성궁경HPV적역감성,AA기인형녀성감염풍험경고,병역우HPV감염적지속화복발.
Objective To investigate the association of interferon (IFN) γ gene polymorphisms and risk and prognosis of HPV cervical infection.Methods PCR-ASP was used for detectiug IFN-γ rs2430561 polymorphism in 179 HPV positive patients and 328 HPV negative normal controls.Results The frequency of A allele of 63.7％ (228/358) was significantly higher than the frequency of T allele of 36.3％ (130/358) in HPV positive group (P =0.045).The frequencies were 41.3％ (74/179) in AA genotype and 14.0％ (25/179) in TT genotype,women carrying AA genotype increased the risk of HPV infection compare with those with TT genotype (OR =1.784,95％ CI:1.031-3.088,P =0.039).During follow-up,the rate of HPV positive again in AA genotype was 83.8％ (62/74),while TT genotype was 20.0％ (5/25).In the analysis of Kaplan-Meier,the cumulative HPV negative rates of AA,TA and TT genotype exhibited significantly different (P =0.008).The cumulative HPV negative rate of AA genotype was the lowest (1.1％-5.9％).Conclusions IFN-γ rs2430561 polymorphisms confer the susceptibility to HPV infection.Women with AA genotype exhibited higher risk of infection and inclined to be continuous status and recurrence after HPV infection.
