中国综合临床
中國綜閤臨床
중국종합림상
CLINICAL MEDICINE OF CHINA
2009年
2期
121-123
,共3页
牛建清%李宏芬%沈志霞%范淑英%代琪%张蕴霞
牛建清%李宏芬%瀋誌霞%範淑英%代琪%張蘊霞
우건청%리굉분%침지하%범숙영%대기%장온하
妊娠期高血压疾病%血管紧张素Ⅱ-1型受体基因%血管紧张素转换酶基因%醛固酮合成酶基因%基因多态性
妊娠期高血壓疾病%血管緊張素Ⅱ-1型受體基因%血管緊張素轉換酶基因%醛固酮閤成酶基因%基因多態性
임신기고혈압질병%혈관긴장소Ⅱ-1형수체기인%혈관긴장소전환매기인%철고동합성매기인%기인다태성
Hypertensive disorder complicating pregnancy%Angiotensin Ⅱ type 1 receptor gene%An-giotensin converting enzyme%Aldosterone synthase gene%Gene polymorphism
目的 探讨血管紧张素Ⅱ-1型受体(AT1R)基因A1166-C、血管紧张素转换酶(ACE)基因插入/缺失(I/D)和醛固酮合成酶(CYP11B2)基因-344T/C位点多态性与妊娠期高血压疾病(HDCP)的相关关系.方法 采用聚合酶链反应-限制性内切酶片段长度多态性技术(PCR-RFLP),分别检测HDCP组86例和正常对照组175例AT1R基因A1166-C、ACE基因I/D和CYP11B2基因-344T/C突变位点的基因型.结果 HDCP组和正常对照组AT1R基因A1166-C、ACE基因I/D和CYPllB2基因-344T/C多态性18种组合的分布不同,构成比不同;这18种组合中,相对于AT1R-AA+ACE-Ⅱ+CYP 1182-TT基因型,携带AT1R-AA+ACE-DD+CYP11B2-TC基因型人群的OR值为7.289;携带AT1R-AC+ACE-ID+CYP11B2-TC基因型人群的OR值为5.315;携带AT1R.AC+ACE-DD+CYP11B2-TC基因型人群的OR值为5.694.其余联合基因型,差异均无统计学意义(P均0.05);或者由于样本量小,不具有代表性.结论 HDCP组和正常对照组AT1R基因A1166-C、ACE基因I/D和CYPllB2基因-344T/C多态性18种组合中,AT1R-AA+ACE-DD+CYP11B2-TC联合基因型、AT1R-AC+ACE-ID+CYP11B2-TC联合基因型、AT1R-AC+ACE-DD+CYP11B2-TC联合基因型可能增加HDCP的遗传易感性;HDCP的发生,可能是多个基因共同作用的结果 .
目的 探討血管緊張素Ⅱ-1型受體(AT1R)基因A1166-C、血管緊張素轉換酶(ACE)基因插入/缺失(I/D)和醛固酮閤成酶(CYP11B2)基因-344T/C位點多態性與妊娠期高血壓疾病(HDCP)的相關關繫.方法 採用聚閤酶鏈反應-限製性內切酶片段長度多態性技術(PCR-RFLP),分彆檢測HDCP組86例和正常對照組175例AT1R基因A1166-C、ACE基因I/D和CYP11B2基因-344T/C突變位點的基因型.結果 HDCP組和正常對照組AT1R基因A1166-C、ACE基因I/D和CYPllB2基因-344T/C多態性18種組閤的分佈不同,構成比不同;這18種組閤中,相對于AT1R-AA+ACE-Ⅱ+CYP 1182-TT基因型,攜帶AT1R-AA+ACE-DD+CYP11B2-TC基因型人群的OR值為7.289;攜帶AT1R-AC+ACE-ID+CYP11B2-TC基因型人群的OR值為5.315;攜帶AT1R.AC+ACE-DD+CYP11B2-TC基因型人群的OR值為5.694.其餘聯閤基因型,差異均無統計學意義(P均0.05);或者由于樣本量小,不具有代錶性.結論 HDCP組和正常對照組AT1R基因A1166-C、ACE基因I/D和CYPllB2基因-344T/C多態性18種組閤中,AT1R-AA+ACE-DD+CYP11B2-TC聯閤基因型、AT1R-AC+ACE-ID+CYP11B2-TC聯閤基因型、AT1R-AC+ACE-DD+CYP11B2-TC聯閤基因型可能增加HDCP的遺傳易感性;HDCP的髮生,可能是多箇基因共同作用的結果 .
목적 탐토혈관긴장소Ⅱ-1형수체(AT1R)기인A1166-C、혈관긴장소전환매(ACE)기인삽입/결실(I/D)화철고동합성매(CYP11B2)기인-344T/C위점다태성여임신기고혈압질병(HDCP)적상관관계.방법 채용취합매련반응-한제성내절매편단장도다태성기술(PCR-RFLP),분별검측HDCP조86례화정상대조조175례AT1R기인A1166-C、ACE기인I/D화CYP11B2기인-344T/C돌변위점적기인형.결과 HDCP조화정상대조조AT1R기인A1166-C、ACE기인I/D화CYPllB2기인-344T/C다태성18충조합적분포불동,구성비불동;저18충조합중,상대우AT1R-AA+ACE-Ⅱ+CYP 1182-TT기인형,휴대AT1R-AA+ACE-DD+CYP11B2-TC기인형인군적OR치위7.289;휴대AT1R-AC+ACE-ID+CYP11B2-TC기인형인군적OR치위5.315;휴대AT1R.AC+ACE-DD+CYP11B2-TC기인형인군적OR치위5.694.기여연합기인형,차이균무통계학의의(P균0.05);혹자유우양본량소,불구유대표성.결론 HDCP조화정상대조조AT1R기인A1166-C、ACE기인I/D화CYPllB2기인-344T/C다태성18충조합중,AT1R-AA+ACE-DD+CYP11B2-TC연합기인형、AT1R-AC+ACE-ID+CYP11B2-TC연합기인형、AT1R-AC+ACE-DD+CYP11B2-TC연합기인형가능증가HDCP적유전역감성;HDCP적발생,가능시다개기인공동작용적결과 .
Objective To explore the relationship among genetic polymorphism of angiotension Ⅱ type 1 re-ceptor(AT1 R) A1166-C, angiotensin converting enzyme (ACE) insertion/deletion (I/D), aldosterone synthase (CYP11B2)-344T/C and hypertensive disorder complicating pregnancy.Methods Polymerase chain reaction-re-striction fragment length polymorphism (PCR-RFLP) assay was used to detect the genotypes of AT1 R A1166-C ,ACE (I/O) ,CYP11B2 -344T/C in 86 cases of hypertensive disorder complicating pregnancy and 175 cases of normal control.Results There was 18 combined types in hypertensive disorder complicating pregnancy cases and normal control cases.Compared to AT1R-AA + ACE-Ⅱ + CYP11B2-TT, Odds ratios (OR) of AT1R-AA + ACE-DO +CYP11B2-TC,AT1 R-AC + ACE-ID+CYP11B2-TC and AT1R-AC+ACE-DD+CYP11B2-TC are 7.289,5.315 and 5.694 respectively.There was no statistical significance among the others.Conclusion In all 18 kinds of combined types, AT1 R-AA + ACE-DO + CYP11B2-TC,AT1R-AC+ACE-ID+CYP11B2-TC and AT1 R-AC + ACE-DD +CYP11B2-TC might increase the susceptibility of hypertensive disorder complicating pregnancy.It is possible that multigenes are interacted in the etiology of hypertensive disorder complicating pregnancy.
