中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2009年
8期
546-549
,共4页
袁明振%赵升田%孟彦%王洪伟%许纯孝
袁明振%趙升田%孟彥%王洪偉%許純孝
원명진%조승전%맹언%왕홍위%허순효
前列腺肿瘤%前列腺增生%受体,毒草碱M3%微血管密度
前列腺腫瘤%前列腺增生%受體,毒草堿M3%微血管密度
전렬선종류%전렬선증생%수체,독초감M3%미혈관밀도
Prostatic neoplasms%Prostatic hyperplasia%Receptor,muscarinic M3%MVD
目的 探讨前列腺组织中胆碱能毒蕈碱受体M3亚型表达在前列腺肿瘤发生发展中的作用.方法 收集正常前列腺、良性前列腺增生、前列腺癌组织标本各36例.应用RT-PCR方法分别测定标本组织中M3受体、血管内皮生长因子(VEGF)的表达情况;蛋白质印迹与免疫组织化学染色方法测定各组标本中M3、VEGF、人白细胞分化抗原34(CD34)的蛋白表达.图像分析系统检测数据,以相对表达强度(目的 物质表达强度/β-actin表达强度)作为统计学分析参数.数据统计分析 计数资料采用方差分析,分类变量资料采用χ2检验,相关性分析采用Pearson方法.结果 前列腺癌组织标本M3受体基因相对表达强度0.8354±0.1897、VEGF基因表达0.7824±0.2047,明显高于良性前列腺增生组织(0.6735±0.1603、0.6021±0.1637)和正常前列腺组织(0.5425±0.1629、0.3436±0.1581),组间差异有统计学意义(P<0.001),3种组织中M3受体与VEGF基因表达呈直线正相关关系(r=0.4999,P<0.001).前列腺癌组织标本M3受体蛋白表达0.4777±0.1638、VEGF蛋白表达0.5718±0.2245,明显高于良性前列腺增生组织(0.3655±0.1474、0.4342±0.1538)和正常前列腺组织(0.2659±0.1076、0.3380±0.1527),组间差异有统计学意义(P<0.05).结论 M3受体表达与前列腺肿瘤的发生发展有关.
目的 探討前列腺組織中膽堿能毒蕈堿受體M3亞型錶達在前列腺腫瘤髮生髮展中的作用.方法 收集正常前列腺、良性前列腺增生、前列腺癌組織標本各36例.應用RT-PCR方法分彆測定標本組織中M3受體、血管內皮生長因子(VEGF)的錶達情況;蛋白質印跡與免疫組織化學染色方法測定各組標本中M3、VEGF、人白細胞分化抗原34(CD34)的蛋白錶達.圖像分析繫統檢測數據,以相對錶達彊度(目的 物質錶達彊度/β-actin錶達彊度)作為統計學分析參數.數據統計分析 計數資料採用方差分析,分類變量資料採用χ2檢驗,相關性分析採用Pearson方法.結果 前列腺癌組織標本M3受體基因相對錶達彊度0.8354±0.1897、VEGF基因錶達0.7824±0.2047,明顯高于良性前列腺增生組織(0.6735±0.1603、0.6021±0.1637)和正常前列腺組織(0.5425±0.1629、0.3436±0.1581),組間差異有統計學意義(P<0.001),3種組織中M3受體與VEGF基因錶達呈直線正相關關繫(r=0.4999,P<0.001).前列腺癌組織標本M3受體蛋白錶達0.4777±0.1638、VEGF蛋白錶達0.5718±0.2245,明顯高于良性前列腺增生組織(0.3655±0.1474、0.4342±0.1538)和正常前列腺組織(0.2659±0.1076、0.3380±0.1527),組間差異有統計學意義(P<0.05).結論 M3受體錶達與前列腺腫瘤的髮生髮展有關.
목적 탐토전렬선조직중담감능독심감수체M3아형표체재전렬선종류발생발전중적작용.방법 수집정상전렬선、량성전렬선증생、전렬선암조직표본각36례.응용RT-PCR방법분별측정표본조직중M3수체、혈관내피생장인자(VEGF)적표체정황;단백질인적여면역조직화학염색방법측정각조표본중M3、VEGF、인백세포분화항원34(CD34)적단백표체.도상분석계통검측수거,이상대표체강도(목적 물질표체강도/β-actin표체강도)작위통계학분석삼수.수거통계분석 계수자료채용방차분석,분류변량자료채용χ2검험,상관성분석채용Pearson방법.결과 전렬선암조직표본M3수체기인상대표체강도0.8354±0.1897、VEGF기인표체0.7824±0.2047,명현고우량성전렬선증생조직(0.6735±0.1603、0.6021±0.1637)화정상전렬선조직(0.5425±0.1629、0.3436±0.1581),조간차이유통계학의의(P<0.001),3충조직중M3수체여VEGF기인표체정직선정상관관계(r=0.4999,P<0.001).전렬선암조직표본M3수체단백표체0.4777±0.1638、VEGF단백표체0.5718±0.2245,명현고우량성전렬선증생조직(0.3655±0.1474、0.4342±0.1538)화정상전렬선조직(0.2659±0.1076、0.3380±0.1527),조간차이유통계학의의(P<0.05).결론 M3수체표체여전렬선종류적발생발전유관.
Objective To study the relationship between M3 receptor and prostastic tumor by analyzing the expressions of M3 and vascular endothelial growth factor(VEGF)in adult human normal and neoplastic prostatic gland tissue. Methods The specimens included 36 normal prostates(fresh),36 benign prostatic hyperplasia(BPH)tissue(fresh),and 36 cancer tissue(8 fresh).RT-PCR was used to detect M3 receptor,VEGPs genetic expression.At protein level,VEGF,Ms receptor,CD34 were detected by western-blot and immunohistochemical method. Results VEGF and M3 receptor's genetic expressions were higher in prostate cancer tissue(O.8354±0.1897,0.7824±0.2047)than in BPH tissue(0.6735±0.1603,0.6021±0.1637),while the expressions of these genes were lowest in normal prostate tissue(0.5425±0.1629,0.3436±0.1581)(P<0.001).There was a positive correlation between M3 and VEGFs gene expression(r=0.4999,P<O.001).The protein expression of Ms receptor and VEGF was similar to their genetic expression pattern,the expressions in prostate cancer tissue(0.4777±0.1638,0.5718±0.2245)were higher than those in BPH tissue(0.3655±0.1474,0.4342±0.1538)and in normal prostate tissue(0.2659±0.1076,0.3380±0.1527)(P<0.05).Conclusions M3 receptor might be related with development of prostatic tumor.It could be used as a new target 0f diagnosis and treatment for prostastic tumor.
