CAJ | 학술논문

目的探讨B7同源物1(B7-H1)分子在同种小鼠角膜移植术后免疫赦免反应中的作用.方法以C57BL/6小鼠为供者,Balb/c小鼠为受者,制备同种小鼠角膜移植.实验分为3组,(1)存活组为术后移植角膜长期存活(＞50d)的受鼠,术后满8周(术后56 d)时取受鼠的移植角膜样本,备检；(2)排斥组为术后50d内发生排斥反应的小鼠,排斥组在判定发生排斥反应时取移植角膜样本,备检；(3)以正常C57BL/6小鼠的角膜样本作为对照组.采用免疫组织化学法和实时荧光定量聚合酶链反应法分别对各组受鼠移植角膜组织内B7-H1蛋白和mRNA的表达水平进行检测,并对B7-H1 mRNA的表达与移植角膜的存活进行相关性分析.结果正常组和存活组小鼠的移植角膜上皮和内皮层均高度表达B7-H1蛋白,排斥组小鼠移植角膜上皮和内皮层B7-H1蛋白的表达均明显减少.存活组、正常组和排斥组受鼠移植角膜组织内B7 H1 mRNA的相对表达量分别为200.0±11.5、44.7±10.8和6.9±12.0,3组间两两比较,差异均有统计学意义(F=241.164,P＜0.01).经相关性分析发现,移植角膜内B7-H1 mRNA表达的高低与移植角膜排斥反应的发生率存在明显的相关性(P＜0.01).结论 B7-H1分子可能介导了同种小鼠角膜移植术后的免疫赦免反应,是维持移植角膜免疫耐受状态的重要因子之一.
목적 탐토B7동원물1(B7-H1)분자재동충소서각막이식술후면역사면반응중적작용.방법 이C57BL/6소서위공자,Balb/c소서위수자,제비동충소서각막이식.실험분위3조,(1)존활조위술후이식각막장기존활(＞50d)적수서,술후만8주(술후56 d)시취수서적이식각막양본,비검；(2)배척조위술후50d내발생배척반응적소서,배척조재판정발생배척반응시취이식각막양본,비검；(3)이정상C57BL/6소서적각막양본작위대조조.채용면역조직화학법화실시형광정량취합매련반응법분별대각조수서이식각막조직내B7-H1단백화mRNA적표체수평진행검측,병대B7-H1 mRNA적표체여이식각막적존활진행상관성분석.결과 정상조화존활조소서적이식각막상피화내피층균고도표체B7-H1단백,배척조소서이식각막상피화내피층B7-H1단백적표체균명현감소.존활조、정상조화배척조수서이식각막조직내B7 H1 mRNA적상대표체량분별위200.0±11.5、44.7±10.8화6.9±12.0,3조간량량비교,차이균유통계학의의(F=241.164,P＜0.01).경상관성분석발현,이식각막내B7-H1 mRNA표체적고저여이식각막배척반응적발생솔존재명현적상관성(P＜0.01).결론 B7-H1분자가능개도료동충소서각막이식술후적면역사면반응,시유지이식각막면역내수상태적중요인자지일.
Objective To clarify the role of B7-H1 in the immune privilege after corneal transplantation in homogeneity variant mice.Methods We established the experimental animal model of allograft mice by using C57BL/6 mouse as donor and Balb/c mouse as recipient.We allocaated the mice with long time survival (＞50 days) corneal graft into survival group,mice with rejection occurring in 50 days into rejection group,and normal C57BL/6 mice into control group.The transplanted corneal grafts were obtained for future reference at the 8th week after transplantation in survival group,and the time of rejection in rejection group.The expression of B7-H1 mRNA was detected by using immunohistochemistry and real time quantitative PCR (RT-PCR),and the relationship between B7-H1 and the immune privilege after corneal transplantation was analyzed. Results The B7 H1 mRNA was highly expressed in epithelium and endothelium of corneal grafts both in survival and control group,in comparison to an obviously lower expression in rejection group.The relative expression level of B7-H1 mRNA was 200.0 ± 11.5 in survival group,44.7 ± 10.8 in control group,and 6.9 ± 12.0 in rejection group,respectively. There were statistically significant differences among the three groups (F=241.164,P＜0.01 ).The was a significant correlation between the level of B7-H1 mRNA and occurrence rate of rejection in corneal graft (P＜0.01 ).Conclusion The results suggest that the immune privilege after corneal transplantation might be mediated by B7-H1,which plays an important role in maintaining the state of corneal immune privilege.