中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2011年
4期
579-581,封3
,共4页
梁朝辉%焦保华%郭二坤%卢圣奎%何心
樑朝輝%焦保華%郭二坤%盧聖奎%何心
량조휘%초보화%곽이곤%로골규%하심
星形细胞瘤%p53%治疗
星形細胞瘤%p53%治療
성형세포류%p53%치료
Astrocytoma%p53%Treatment
目的 探讨野生型p53-诱导的蛋白磷酸酶1(Wip1)在人脑星形细胞瘤组织中的表达及其临床意义.方法 应用免疫组织化学、逆转录-聚合酶链反应(RT-PCR)和Western blot方法 检测32例高级别星形细胞瘤、35例低级别星形细胞瘤及10例正常脑组织中Wip1蛋白、mRNA表达,对Wip1阳性表达组织进行p53免疫组织化学和Western blot检测;同时分析Wip1表达与临床病理因素之间的关系.结果 免疫组织化学:高级别星形细胞瘤、低级别星形细胞瘤和正常脑组织中Wip1阳性表达率分别为24/32(75.0%)、11/35(31.4%)、1/10(10.0%),高级别星形细胞瘤Wip1表达高于低级别星形细胞瘤及正常脑组织,差异有统计学意义(P＜0.05).RT-PCR:Wip1 mRNA在高级别星形细胞瘤、低级别星形细胞瘤和正常脑组织表达相对量分别为:0.35±0.07、0.07±0.03、0.05±0.02,高级别星形细胞瘤Wip1 mRNA高于低级别星形细胞瘤及正常脑组织,差异有统计学意义(P＜0.05).Wip1 Western blot显示:高级别、低级别星形细胞瘤和正常脑组织表达相对量1.67±0.12、1.05±0.25、0.41±0.16,高级别高于低级别及正常脑组织的表达,差异有统计学意义(P＜0.05).对其中36例Wip1阳性表达组织进行p53免疫组织化学染色,13例呈阳性表达,p53与Wip1表达呈负相关(r=-0.29).p53 Western blot显示:高级别、低级别星形细胞瘤及正常脑组织中表达相对量为:0.77±0.09、0.63±0.13、0.21±0.11,高级别组和低级别组高于正常脑组织组,差异有统计学意义(P＜0.05),高级别组与低级别组差异无统计学意义(P＞0.05).Wip1与患者年龄、肿瘤大小无明显相关(P＞0.05).结论 Wip1在高级别星形细胞瘤高表达,与肿瘤病理级别呈正相关,与p53表达呈负相关,可能与肿瘤恶性生长有关.
目的 探討野生型p53-誘導的蛋白燐痠酶1(Wip1)在人腦星形細胞瘤組織中的錶達及其臨床意義.方法 應用免疫組織化學、逆轉錄-聚閤酶鏈反應(RT-PCR)和Western blot方法 檢測32例高級彆星形細胞瘤、35例低級彆星形細胞瘤及10例正常腦組織中Wip1蛋白、mRNA錶達,對Wip1暘性錶達組織進行p53免疫組織化學和Western blot檢測;同時分析Wip1錶達與臨床病理因素之間的關繫.結果 免疫組織化學:高級彆星形細胞瘤、低級彆星形細胞瘤和正常腦組織中Wip1暘性錶達率分彆為24/32(75.0%)、11/35(31.4%)、1/10(10.0%),高級彆星形細胞瘤Wip1錶達高于低級彆星形細胞瘤及正常腦組織,差異有統計學意義(P＜0.05).RT-PCR:Wip1 mRNA在高級彆星形細胞瘤、低級彆星形細胞瘤和正常腦組織錶達相對量分彆為:0.35±0.07、0.07±0.03、0.05±0.02,高級彆星形細胞瘤Wip1 mRNA高于低級彆星形細胞瘤及正常腦組織,差異有統計學意義(P＜0.05).Wip1 Western blot顯示:高級彆、低級彆星形細胞瘤和正常腦組織錶達相對量1.67±0.12、1.05±0.25、0.41±0.16,高級彆高于低級彆及正常腦組織的錶達,差異有統計學意義(P＜0.05).對其中36例Wip1暘性錶達組織進行p53免疫組織化學染色,13例呈暘性錶達,p53與Wip1錶達呈負相關(r=-0.29).p53 Western blot顯示:高級彆、低級彆星形細胞瘤及正常腦組織中錶達相對量為:0.77±0.09、0.63±0.13、0.21±0.11,高級彆組和低級彆組高于正常腦組織組,差異有統計學意義(P＜0.05),高級彆組與低級彆組差異無統計學意義(P＞0.05).Wip1與患者年齡、腫瘤大小無明顯相關(P＞0.05).結論 Wip1在高級彆星形細胞瘤高錶達,與腫瘤病理級彆呈正相關,與p53錶達呈負相關,可能與腫瘤噁性生長有關.
목적 탐토야생형p53-유도적단백린산매1(Wip1)재인뇌성형세포류조직중적표체급기림상의의.방법 응용면역조직화학、역전록-취합매련반응(RT-PCR)화Western blot방법 검측32례고급별성형세포류、35례저급별성형세포류급10례정상뇌조직중Wip1단백、mRNA표체,대Wip1양성표체조직진행p53면역조직화학화Western blot검측;동시분석Wip1표체여림상병리인소지간적관계.결과 면역조직화학:고급별성형세포류、저급별성형세포류화정상뇌조직중Wip1양성표체솔분별위24/32(75.0%)、11/35(31.4%)、1/10(10.0%),고급별성형세포류Wip1표체고우저급별성형세포류급정상뇌조직,차이유통계학의의(P＜0.05).RT-PCR:Wip1 mRNA재고급별성형세포류、저급별성형세포류화정상뇌조직표체상대량분별위:0.35±0.07、0.07±0.03、0.05±0.02,고급별성형세포류Wip1 mRNA고우저급별성형세포류급정상뇌조직,차이유통계학의의(P＜0.05).Wip1 Western blot현시:고급별、저급별성형세포류화정상뇌조직표체상대량1.67±0.12、1.05±0.25、0.41±0.16,고급별고우저급별급정상뇌조직적표체,차이유통계학의의(P＜0.05).대기중36례Wip1양성표체조직진행p53면역조직화학염색,13례정양성표체,p53여Wip1표체정부상관(r=-0.29).p53 Western blot현시:고급별、저급별성형세포류급정상뇌조직중표체상대량위:0.77±0.09、0.63±0.13、0.21±0.11,고급별조화저급별조고우정상뇌조직조,차이유통계학의의(P＜0.05),고급별조여저급별조차이무통계학의의(P＞0.05).Wip1여환자년령、종류대소무명현상관(P＞0.05).결론 Wip1재고급별성형세포류고표체,여종류병리급별정정상관,여p53표체정부상관,가능여종류악성생장유관.
Objective To explore the expression of wild-type p53-induced phosphatase 1 (Wip1)in astrocytomas and its clinical significance. Methods In 32 cases of high-grade astrocytomas, 35 cases of low-grade astrocytomas and 10 cases of normal brain tissues the expression of Wip1 mRNA and protein was detected by using immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR)and Western blotting. The expression of p53 was assayed in Wip1 positive tissues by using immunohistochemistry and Western blotting. The relationship between the Wip1 expression and clinicopathological features was analyzed. Results ( 1 ) Immunohistochemistry : The positve expression rate of Wip1 in highgrade astrocytoma, low-grade astrocytoma and normal brain tissues was 24/32 (75.0%), 11/35(31.4%) and 1/10 (10.0%) respectively. There was significant difference among three tissues (P＜0. 05 ). (2) RT-PCR: The expression level of Wip1 mRNA in high-grade astrocytoma, low-grade astrocytoma and normal brain tissues was 0. 35 ±0. 07, 0. 07±0. 03 and 0. 05 ±0. 02 respectively. The expression level of Wip1 mRNA in high-grade astrocytoma was higher than that in low-grade astrocytoma and normal brain tissues (P ＜0. 05). (3) Western blotting: The relative expression of Wipl protein in high-grade astrocytoma, low-grade astrocytoma and normal brain tissues was 1.67 ±0. 12, 1.05 ±0.25 and 0.41 ±0. 16 respectively, p53 immunohistochemical staining revealed that only 13 were positive for p53 in 36Wip1 positive tissues. The high expression rate of Wip1 protein was negatively correlated with the expression of p53 ( r = - 0. 29). The relative expression of p53 protein in high-grade astrocytoma, low-grade astrocytoma and normal brain tissues was 0. 77 ±0. 09, 0. 63 ±0. 13 and 0. 21 ±0. 11 respectively. They was no statistically significant difference between high-grade astrocytoma and low-grade astrocytoma (P ＞0. 05), but the relative expression of p53 protein in high-grade astrocytoma, low-grade astrocytoma was higher than in normal brain tissues ( P ＜ 0. 05 ). The expression of Wip1 was greatly correlated with its pathologic classification ( P ＜ 0. 05 ), but it had nothing to do with tumor size and age ( P ＞ 0. 05 ). Conclusion The high expression of Wipl mRNA and protein in astrocytoma is related to its pathologic classification and negatively correlated with the expression of p53. Wip1 may play an important role in the tumor development.
